Table of Contents

1. Evaluation of I-TASSER/COFACTOR on 25 newly annotated neXtProt proteins 2. Methods and overall results for 44 neXtProt proteins Technical note on the use of UniProt proteins as templates for function prediction



1. Evaluation of I-TASSER/COFACTOR on 25 newly annotated neXtProt proteins

As of the neXtProt release 2019-01-11, 25 previously uPE1 proteins acquired new function annotations (out of 44 submitted for such curation). We earlier blindly analyzed and submitted our predictions for all 44 (see Table 3 in Section 2.). In this section, we closely compare the results for the 25.

Among the 25 targets, our method did not assign free-text function annotation for three of them (O75363-1, Q8NDM7-1, Q9BZH6-1), even though we did predict their GO terms. For the remaining 22 targets, our manual function interpretation are consistent with neXtProt annotation for 9 of them. A list of the accessions, our free text annotations, and neXtProt 2019-01-11 free text annotation are summarized in the first, third, and fifth columns in Table 1, respectively, where targets whose manual function interpretation is consistent with neXtProt free text annotation are marked by asterisks (*) in the second column. Meanwhile, another spreadsheet listing the our free text annotation and the templates (structure templates, blast/psiblast hits, and PPI partners) are available in plain text (25.tsv) and Excel (25.xls) format.


Free text function annotations, either those assigned based on I-TASSER/COFACTOR prediction or those assigned by neXtProt curators based on existing literature, could potentially include bias in manual intepretation of function prediction results or literature. Therefore, performance of function prediction should also be quantitatively evaluated by correctness of GO term annotation. For the 25 targets with new neXtProt function annotation, 8 and 21 of them acquired GO terms with "Gold" status for MF and BP aspects, respectively, excluding GO annotations deemed by neXtProt as being too general (https://www.nextprot.org/proteins/search?mode=advanced&queryId=NXQ_00022), such as "protein binding", "metal ion binding", "calcium ion binding". The list of the 13 excluded GO terms is available here. We list the specific GO terms newly assigned by neXtProt and predicted by COFACTOR predicted GO terms in Table 2. Three targets (Q6AI39-1, Q96J88-1, Q9BZD6-1) do not acquire any specific GO terms in neXtProt version 2019-01-11 despite new free text annotation. For each of the remaining 22 targets, we evaluate the performance of GO term prediction by maximum F-measure, also known as F1 score, defined as the harmonic average of precision and recall:
where TP, FP, FN are the number of correctly predicted GO terms, incorrectly predicted GO terms, and GO terms annotated by neXtProt but missed by our predictor. The values of TP, FP, and FN are depedent on C-score cutoff. Therefore, we test all C-score cutoff from 0 to 1 and report the maximum F-measure. Since neXtProt consider MF and BP but not CC as function annotation, F-measures are calculated separately for MF and BP only. The F-measures for MF/BP are shown in the fourth column in the following table. The target with inconsistent free text annotations but appreciable GO term prediction accuracy (F-measure>0.5 for either MF or BP) is marked by the plus sign (+) in the first column.

We note that free text consistency and Fmax does not always correlates with each other. This is in part because, in many scenarios, one protein can perform multiple functions. For example, target O75677 regulates cell cycle by promoting ubquitin-dependent protein degradation. While our MF prediction can infer the ubquitin-dependent protein degradation function, our BP term prediction cannot correctly predict the cell cycle regulation function, resulting in a low Fmax despite partially consistent function annotation.

Table 1. Comparison of free-text function annotation by I-TASSER/COFACTOR and by neXtProt curation for 25 uPE1 proteins with newly provided function annotation in release 2019-01-11.

a An asterisk (*) marks a target if our free-text annotation (see below) matches
  neXtProt free text annotation. For each target, we manually assign a free-text annotation
  based on specific GO term predicted by our automatic I-TASSER/COFACTOR pipeline.
b A plus (+) marks a target whose Fmax (see above) for either MF or BP is >0.5
  Fmax for MF/BP quantitatively measures the consistency between COFACTOR predicted GO terms
  and neXtProt curated GO terms. "NA" means neXtProt did not assign GO term for a target.
#Accession, GeneMatcha,bOur annotation and structure modelFmax MF/BPneXtProt annotation
1Q96M27-1, PRRC1*protein kinase A regulation
1.00,0.88Activation of protein kinase A activity. Protein binding. Protein kinase A regulatory subunit binding.
2P0C870-1, JMJD7*histone demethylation
0.55,0.90Bifunctional enzyme that acts both as an endopeptidase and 2-oxoglutarate-dependent monoxygenase. Endopeptidase that cleaves histones N-terminal tails at the carboxyl side of methylated arginine or lysine residues, to generate 'tailless nucleosomes', which may trigger transcription elongation. Preferentially recognizes and cleaves monomethylated and dimethylated arginine residues of histones H2, H3 and H4. After initial cleavage, continues to digest histones tails via its aminopeptidase activity. Additionally, may play a role in protein biosynthesis by modifying the translation machinery. Acts as Fe2+ and 2-oxoglutarate-dependent monoxygenase, catalyzing (S)-stereospecific hydroxylation at C-3 of 'Lys-22' of DRG1 and 'Lys-21' of DRG2 translation factors (TRAFAC), promoting their interaction with ribonucleic acids (RNA).
3Q7Z5A7-1, FAM19A5*regulation of microglial cell activation
0.67,0.50Acts as a chemokine-like protein by regulating cell proliferation and migration through activation of G protein-coupled receptors (GPCRs), such as S1PR2 and FPR2. Stimulates chemotactic migration of macrophages mediated by the MAPK3/ERK1 and AKT1 pathway. Blocks TNFSF11/RANKL-induced osteoclast formation from macrophages by inhibiting up-regulation of osteoclast fusogenic and differentiation genes. Stimulation of macrophage migration and inhibition of osteoclast formation is mediated via GPCR FPR2. Acts as an adipokine by negatively regulating vascular smooth muscle cell (VSMC) proliferation and migration in response to platelet-derived growth factor stimulation via GPCR S1PR2 and G protein GNA12/GNA13-transmitted RHOA signaling. Inhibits injury-induced cell proliferation and neointima formation in the femoral arteries
4Q5T0D9-1, TPRG1L+phosphatidylinositol-4-phosphate phosphatase
NA,0.55Presynaptic protein involved in the synaptic transmission tuning. Regulates synaptic release probability by decreasing the calcium sensitivity of release
5Q96D15-1, RCN3catalytic activity, acting on a protein
NA,0.47Probable molecular chaperone assisting protein biosynthesis and transport in the endoplasmic reticulum. Required for the proper biosynthesis and transport of pulmonary surfactant-associated protein A/SP-A, pulmonary surfactant-associated protein D/SP-D and the lipid transporter ABCA3. By regulating both the proper expression and the degradation through the endoplasmic reticulum-associated protein degradation pathway of these proteins plays a crucial role in pulmonary surfactant homeostasis. Has an anti-fibrotic activity by negatively regulating the secretion of type I and type III collagens. This calcium-binding protein also transiently associates with immature PCSK6 and regulates its secretion.
6Q8WTR8-1, NTN5*anatomical structure morphogenesis
NA,0.44Plays a role in neurogenesis. Prevents motor neuron cell body migration out of the neural tube.
7Q9C0D6-1, FHDC1*binding of cytoskeleton
0.44,0.33Microtubule-associated formin which regulates both actin and microtubule dynamics. Induces microtubule acetylation and stabilization and actin stress fiber formation. Regulates Golgi ribbon formation. Required for normal cilia assembly.
8O75363-1, BCAS1(for CC: neuron part)
NA,0.40Required for myelination.
9P60827-1, C1QTNF8*signaling receptor binding
NA,0.40May play a role as ligand of relaxin receptor RXFP1.
10Q8IUY3-1, GRAMD2Abinding of GTPase from Ras superfamily
0.11,0.38Participates in the organization of endoplasmic reticulum-plasma membrane contact sites (EPCS) with pleiotropic functions including STIM1 recruitment and calcium homeostasis. Constitutive tether that co-localize with ESYT2/3 tethers at endoplasmic reticulum-plasma membrane contact sites in a phosphatidylinositol lipid-dependent manner. Pre-marks the subset of phosphtidylinositol 4,5-biphosphate (PI(4,5)P2)-enriched EPCS destined for the store operated calcium entry pathway (SOCE).
11Q9BZH6-1, WDR11
NA,0.35Involved in the Hedgehog (Hh) signaling pathway, is essential for normal ciliogenesis. Regulates the proteolytic processing of GLI3 and cooperates with the transcription factor EMX1 in the induction of downstream Hh pathway gene expression and gonadotropin-releasing hormone production. WDR11 complex facilitates the tethering of Adaptor protein-1 complex (AP-1)-derived vesicles. WDR11 complex acts together with TBC1D23 to facilitate the golgin-mediated capture of vesicles generated using AP-1.
12Q6ZNE9-2, RUFY4regulation of protein folding
0.26,0.32Positively regulates macroautophagy in primary dendritic cells. Increases autophagic flux, probably by stimulating both autophagosome formation and facilitating tethering with lysosomes. Binds to phosphatidylinositol 3-phosphate (PtdIns3P) through its FYVE-type zinc finger.
13Q9GZU8-1, FAM192Ahydrolase, probably hydrolase of protein
NA,0.32Promotes the association of the proteasome activator complex subunit PSME3 with the 20S proteasome and regulates its activity. Inhibits PSME3-mediated degradation of some proteasome substrates, probably by affecting their diffusion rate into the catalytic chamber of the proteasome
14Q494U1-1, PLEKHN1transmembrane transport of small molecules, such as nucleotide
0.05,0.29Controls the stability of the leptin mRNA harboring an AU-rich element (ARE) in its 3' UTR, in cooperation with the RNA stabilizer ELAVL1
15Q8IUW5-1, RELL1*regulation of apoptosis through TNF
NA,0.29Induces activation of MAPK14/p38 cascade, when overexpressed.
16Q8NDM7-1, CFAP43
NA,0.29Flagellar protein involved in sperm flagellum axoneme organization and function.
17Q8TDG2-1, ACTRT1regulation of chromosome organization either though histone acetylation or binding of cytoskelton used in chromsome segregation
0.03,0.29Negatively regulates the Hedgehog (SHH) signaling. Binds to the promoter of the SHH signaling mediator, GLI1, and inhibits its expression.
18O75677-1, RFPL1*ubiquitin-protein transferase activity
NA,0.27Negatively regulates the G2-M phase transition, possibly by promoting cyclin B1/CCNB1 and CDK1 proteasomal degradation and thereby preventing their accumulation during interphase.
19Q5VTQ0-1, TTC39B*protein ubiquitination regulation
NA,0.26Regulates high density lipoprotein (HDL) cholesterol metabolism by promoting the ubiquitination and degradation of the oxysterols receptors LXR (NR1H2 and NR1H3).
20Q96S16-1, JMJD8histone demethylation
NA,0.21Functions as a positive regulator of TNF-induced NF-kappa-B signaling. Regulates angiogenesis and cellular metabolism through interaction with PKM.
21Q9H9L7-1, AKIRIN1by binding to RNA polymerase, regulate expression of genes such as cytokines
NA,0.18Functions as signal transducer for MSTN during skeletal muscle regeneration and myogenesis. May regulates chemotaxis of both macrophages and myoblasts by reorganising actin cytoskeleton, leading to more efficient lamellipodia formation via a PI3 kinase dependent pathway.
22Q96KV7-1, WDR90regulation of transcription by nucleic acid binding
NA,0.17Required for efficient primary cilium formation.
23Q6AI39-1, BICRALsodium:potassium ion transporter
NA,NAComponent of SWI/SNF chromatin remodeling subcomplex GBAF that carries out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner.
24Q96J88-1, EPSTI1cytoskeleton binding
NA,NAPlays a role in M1 macrophage polarization and is required for the proper regulation of gene expression during M1 versus M2 macrophage differentiation. Might play a role in RELA/p65 and STAT1 phosphorylation and nuclear localization upon activation of macrophages.
25Q9BZD6-1, PRRG4serine-type endopeptidase
NA,NAMay control axon guidance across the CNS. Prevents the delivery of ROBO1 at the cell surface and downregulates its expression.

Table 2. Comparison of GO term annotation by I-TASSER/COFACTOR and by neXtProt curation for 25 uPE1 proteins with newly provided function annotation in release 2019-01-11.

a An asterisk (*) marks a target if our free-text annotation (see below) matches neXtProt
  free text annotation. A plus (+) marks a target whose Fmax (see above) for either MF or BP is >0.5
b For each target, we manually assign a free-text annotation based on specific GO term
  predicted by our automatic I-TASSER/COFACTOR pipeline.  Additionally, we show, in a directed acyclic
  graph (DAG), the predicted GO terms used to calculate the Fmax. GO terms matching neXtProt annotations
  are colored in red. Root terms ("molecular_function" and "biological_process") and "protein binding"
  terms are not used to calculate Fmax, as per CAFA standard. Click on the image to get an enlarge
  version. If neXtProt did not assign GO terms, the DAG is not shown. Additionally, the I-TASSER
  structure model is available for download by clicking on the structure image.
c Fmax for MF/BP quantitatively measures the consistency between COFACTOR predicted GO terms
  and neXtProt curated GO terms. "NA" means neXtProt did not assign GO term for a target.
d neXtProt GO terms covered by I-TASSER/COFACTOR annotations are colored in red in the graph.
#Accession, Gene (chromosome)MatchaOur annotationbFmax MF/BPcneXtProt annotationd
1 Q96M27-1, PRRC1 (Chr5) * protein kinase A regulation
==== MF ====
GO:0034237 F 0.65 protein kinase A regulatory subunit binding

==== BP ====
GO:0065007 P 0.94 biological regulation
GO:0050789 P 0.92 regulation of biological process
GO:0050794 P 0.91 regulation of cellular process
GO:0060255 P 0.82 regulation of macromolecule metabolic process
GO:0031323 P 0.81 regulation of cellular metabolic process
GO:0080090 P 0.80 regulation of primary metabolic process
GO:0051171 P 0.80 regulation of nitrogen compound metabolic process
GO:0048518 P 0.79 positive regulation of biological process
GO:0048522 P 0.77 positive regulation of cellular process
GO:0009987 P 0.77 cellular process
GO:0065008 P 0.74 regulation of biological quality
GO:0032501 P 0.74 multicellular organismal process
GO:0010604 P 0.73 positive regulation of macromolecule metabolic process
GO:0051173 P 0.72 positive regulation of nitrogen compound metabolic process
GO:0031325 P 0.72 positive regulation of cellular metabolic process
GO:0065009 P 0.71 regulation of molecular function
GO:0051246 P 0.71 regulation of protein metabolic process
GO:0032268 P 0.70 regulation of cellular protein metabolic process
GO:0050790 P 0.69 regulation of catalytic activity
GO:0031399 P 0.69 regulation of protein modification process
GO:0051247 P 0.68 positive regulation of protein metabolic process
GO:0044093 P 0.68 positive regulation of molecular function
GO:0019220 P 0.68 regulation of phosphate metabolic process
GO:0045859 P 0.67 regulation of protein kinase activity
GO:0042592 P 0.67 homeostatic process
GO:0001934 P 0.67 positive regulation of protein phosphorylation
GO:0043085 P 0.66 positive regulation of catalytic activity
GO:0007389 P 0.66 pattern specification process
GO:0034199 P 0.65 activation of protein kinase A activity
GO:0010669 P 0.65 epithelial structure maintenance
GO:0001757 P 0.65 somite specification

 1.00,0.88 Activation of protein kinase A activity. Protein binding. Protein kinase A regulatory subunit binding.
==== MF ====
GO:0034237 ! protein kinase A regulatory subunit binding

==== BP ====
GO:0034199 ! activation of protein kinase A activity
2 P0C870-1, JMJD7 (Chr15) * histone demethylation
==== MF ====
GO:0003824 F 0.98 catalytic activity
GO:0016491 F 0.79 oxidoreductase activity
GO:0016705 F 0.78 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0140096 F 0.77 catalytic activity, acting on a protein
GO:0016706 F 0.76 2-oxoglutarate-dependent dioxygenase activity
GO:1901363 F 0.69 heterocyclic compound binding
GO:0097159 F 0.69 organic cyclic compound binding
GO:0032452 F 0.63 histone demethylase activity
GO:0043167 F 0.56 ion binding
GO:0046872 F 0.52 metal ion binding

==== BP ====
GO:0009987 P 1.00 cellular process
GO:0008152 P 0.98 metabolic process
GO:0071704 P 0.97 organic substance metabolic process
GO:0044238 P 0.97 primary metabolic process
GO:0044237 P 0.97 cellular metabolic process
GO:1901564 P 0.96 organonitrogen compound metabolic process
GO:0043412 P 0.95 macromolecule modification
GO:0044260 P 0.84 cellular macromolecule metabolic process
GO:0065007 P 0.78 biological regulation
GO:0050789 P 0.77 regulation of biological process
GO:0006464 P 0.76 cellular protein modification process

 0.55,0.90 Bifunctional enzyme that acts both as an endopeptidase and 2-oxoglutarate-dependent monoxygenase. Endopeptidase that cleaves histones N-terminal tails at the carboxyl side of methylated arginine or lysine residues, to generate 'tailless nucleosomes', which may trigger transcription elongation. Preferentially recognizes and cleaves monomethylated and dimethylated arginine residues of histones H2, H3 and H4. After initial cleavage, continues to digest histones tails via its aminopeptidase activity. Additionally, may play a role in protein biosynthesis by modifying the translation machinery. Acts as Fe2+ and 2-oxoglutarate-dependent monoxygenase, catalyzing (S)-stereospecific hydroxylation at C-3 of 'Lys-22' of DRG1 and 'Lys-21' of DRG2 translation factors (TRAFAC), promoting their interaction with ribonucleic acids (RNA).
==== MF ====
GO:0016706 ! 2-oxoglutarate-dependent dioxygenase activity
GO:0004177 ! aminopeptidase activity
GO:0004175 ! endopeptidase activity
GO:0046872 ! metal ion binding
GO:0035064 ! methylated histone binding
GO:0004497 ! monooxygenase activity

==== BP ====
GO:0018126 ! protein hydroxylation
3 Q7Z5A7-1, FAM19A5 (Chr22) * regulation of microglial cell activation
==== MF ====


==== BP ====
GO:0050789 P 1.00 regulation of biological process
GO:0050794 P 0.85 regulation of cellular process
GO:0009987 P 0.84 cellular process
GO:0048522 P 0.75 positive regulation of cellular process
GO:0048583 P 0.73 regulation of response to stimulus

 0.67,0.50 Acts as a chemokine-like protein by regulating cell proliferation and migration through activation of G protein-coupled receptors (GPCRs), such as S1PR2 and FPR2. Stimulates chemotactic migration of macrophages mediated by the MAPK3/ERK1 and AKT1 pathway. Blocks TNFSF11/RANKL-induced osteoclast formation from macrophages by inhibiting up-regulation of osteoclast fusogenic and differentiation genes. Stimulation of macrophage migration and inhibition of osteoclast formation is mediated via GPCR FPR2. Acts as an adipokine by negatively regulating vascular smooth muscle cell (VSMC) proliferation and migration in response to platelet-derived growth factor stimulation via GPCR S1PR2 and G protein GNA12/GNA13-transmitted RHOA signaling. Inhibits injury-induced cell proliferation and neointima formation in the femoral arteries
==== MF ====
GO:0005125 ! cytokine activity

==== BP ====
GO:0010469 ! regulation of signaling receptor activity
4 Q5T0D9-1, TPRG1L (Chr1) + phosphatidylinositol-4-phosphate phosphatase
==== BP ====
GO:0065007 P 0.96 biological regulation
GO:0050794 P 0.95 regulation of cellular process

 NA,0.55 Presynaptic protein involved in the synaptic transmission tuning. Regulates synaptic release probability by decreasing the calcium sensitivity of release
==== BP ====
GO:0051966 ! regulation of synaptic transmission, glutamatergic
5 Q96D15-1, RCN3 (Chr19) catalytic activity, acting on a protein
==== BP ====
GO:0009987 P 0.95 cellular process
GO:0065007 P 0.83 biological regulation
GO:0050789 P 0.78 regulation of biological process
GO:0050794 P 0.75 regulation of cellular process
GO:0071704 P 0.66 organic substance metabolic process
GO:0044237 P 0.65 cellular metabolic process
GO:0044238 P 0.64 primary metabolic process
GO:0006807 P 0.63 nitrogen compound metabolic process
GO:0043170 P 0.61 macromolecule metabolic process
GO:0044260 P 0.56 cellular macromolecule metabolic process
GO:1901564 P 0.54 organonitrogen compound metabolic process
GO:0007165 P 0.54 signal transduction
GO:0019538 P 0.52 protein metabolic process
GO:0043412 P 0.51 macromolecule modification

 NA,0.47 Probable molecular chaperone assisting protein biosynthesis and transport in the endoplasmic reticulum. Required for the proper biosynthesis and transport of pulmonary surfactant-associated protein A/SP-A, pulmonary surfactant-associated protein D/SP-D and the lipid transporter ABCA3. By regulating both the proper expression and the degradation through the endoplasmic reticulum-associated protein degradation pathway of these proteins plays a crucial role in pulmonary surfactant homeostasis. Has an anti-fibrotic activity by negatively regulating the secretion of type I and type III collagens. This calcium-binding protein also transiently associates with immature PCSK6 and regulates its secretion.
==== BP ====
GO:0009306 ! protein secretion
GO:0010952 ! positive regulation of peptidase activity
GO:0015031 ! protein transport
GO:0032964 ! collagen biosynthetic process
GO:0036503 ! ERAD pathway
GO:0043129 ! surfactant homeostasis
GO:0051896 ! regulation of protein kinase B signaling
GO:0055091 ! phospholipid homeostasis
GO:0060428 ! lung epithelium development
6 Q8WTR8-1, NTN5 (Chr19) * anatomical structure morphogenesis
==== BP ====
GO:0009987 P 0.98 cellular process
GO:0065007 P 0.88 biological regulation
GO:0050789 P 0.86 regulation of biological process
GO:0032502 P 0.86 developmental process

 NA,0.44 Plays a role in neurogenesis. Prevents motor neuron cell body migration out of the neural tube.
==== BP ====
GO:0022008 ! neurogenesis
7 Q9C0D6-1, FHDC1 (Chr4) * binding of cytoskeleton
==== MF ====
GO:0008092 F 0.70 cytoskeletal protein binding
GO:0003824 F 0.60 catalytic activity

==== BP ====
GO:0009987 P 0.95 cellular process
GO:0065007 P 0.82 biological regulation
GO:0050789 P 0.80 regulation of biological process
GO:0050794 P 0.75 regulation of cellular process
GO:0016043 P 0.69 cellular component organization
GO:0032502 P 0.54 developmental process

 0.44,0.33 Microtubule-associated formin which regulates both actin and microtubule dynamics. Induces microtubule acetylation and stabilization and actin stress fiber formation. Regulates Golgi ribbon formation. Required for normal cilia assembly.
==== MF ====
GO:0003779 ! actin binding
GO:0008017 ! microtubule binding

==== BP ====
GO:0043149 ! stress fiber assembly
GO:0060271 ! cilium assembly
GO:0090161 ! Golgi ribbon formation
8 O75363-1, BCAS1 (Chr20) (for CC: neuron part)
==== BP ====
GO:0009987 P 0.90 cellular process

 NA,0.40 Required for myelination.
==== BP ====
GO:0042552 ! myelination
9 P60827-1, C1QTNF8 (Chr16) * signaling receptor binding
==== BP ====
GO:0009987 P 0.93 cellular process
GO:0065007 P 0.85 biological regulation
GO:0050789 P 0.82 regulation of biological process
GO:0048518 P 0.67 positive regulation of biological process
GO:0032502 P 0.65 developmental process
GO:0050896 P 0.62 response to stimulus
GO:0050794 P 0.62 regulation of cellular process
GO:0048583 P 0.62 regulation of response to stimulus

 NA,0.40 May play a role as ligand of relaxin receptor RXFP1.
==== BP ====
GO:2000147 ! positive regulation of cell motility
10 Q8IUY3-1, GRAMD2A (Chr15) binding of GTPase from Ras superfamily
==== MF ====
GO:0098772 F 0.64 molecular function regulator
GO:0017016 F 0.63 Ras GTPase binding
GO:0005096 F 0.62 GTPase activator activity
GO:0017137 F 0.61 Rab GTPase binding

==== BP ====
GO:0065007 P 0.85 biological regulation
GO:0051179 P 0.84 localization
GO:0051641 P 0.83 cellular localization
GO:0050794 P 0.82 regulation of cellular process

 0.11,0.38 Participates in the organization of endoplasmic reticulum-plasma membrane contact sites (EPCS) with pleiotropic functions including STIM1 recruitment and calcium homeostasis. Constitutive tether that co-localize with ESYT2/3 tethers at endoplasmic reticulum-plasma membrane contact sites in a phosphatidylinositol lipid-dependent manner. Pre-marks the subset of phosphtidylinositol 4,5-biphosphate (PI(4,5)P2)-enriched EPCS destined for the store operated calcium entry pathway (SOCE).
==== MF ====
GO:0005546 ! phosphatidylinositol-4,5-bisphosphate binding
GO:0035091 ! phosphatidylinositol binding

==== BP ====
GO:0061817 ! endoplasmic reticulum-plasma membrane tethering
GO:2001256 ! regulation of store-operated calcium entry
11 Q9BZH6-1, WDR11 (Chr10)
==== BP ====
GO:0009987 P 0.97 cellular process
GO:0065007 P 0.85 biological regulation
GO:0050789 P 0.82 regulation of biological process
GO:0050794 P 0.80 regulation of cellular process
GO:0044237 P 0.75 cellular metabolic process
GO:0071704 P 0.73 organic substance metabolic process
GO:0044238 P 0.73 primary metabolic process
GO:0071840 P 0.72 cellular component organization or biogenesis
GO:0006807 P 0.72 nitrogen compound metabolic process
GO:0043170 P 0.71 macromolecule metabolic process
GO:0016043 P 0.69 cellular component organization
GO:0048518 P 0.63 positive regulation of biological process
GO:0019222 P 0.62 regulation of metabolic process
GO:0060255 P 0.61 regulation of macromolecule metabolic process
GO:0031323 P 0.61 regulation of cellular metabolic process
GO:0080090 P 0.60 regulation of primary metabolic process
GO:0051171 P 0.60 regulation of nitrogen compound metabolic process
GO:0048522 P 0.59 positive regulation of cellular process
GO:0050896 P 0.58 response to stimulus
GO:1901360 P 0.56 organic cyclic compound metabolic process
GO:0044260 P 0.56 cellular macromolecule metabolic process
GO:0034641 P 0.56 cellular nitrogen compound metabolic process
GO:0032502 P 0.56 developmental process
GO:0006725 P 0.56 cellular aromatic compound metabolic process
GO:0046483 P 0.55 heterocycle metabolic process
GO:0022607 P 0.55 cellular component assembly
GO:0090304 P 0.54 nucleic acid metabolic process
GO:0007165 P 0.54 signal transduction
GO:0019219 P 0.53 regulation of nucleobase-containing compound metabolic process
GO:0048583 P 0.51 regulation of response to stimulus
GO:0009893 P 0.51 positive regulation of metabolic process
GO:0051173 P 0.50 positive regulation of nitrogen compound metabolic process
GO:0043933 P 0.50 protein-containing complex subunit organization
GO:0031325 P 0.50 positive regulation of cellular metabolic process
GO:0010604 P 0.50 positive regulation of macromolecule metabolic process

 NA,0.35 Involved in the Hedgehog (Hh) signaling pathway, is essential for normal ciliogenesis. Regulates the proteolytic processing of GLI3 and cooperates with the transcription factor EMX1 in the induction of downstream Hh pathway gene expression and gonadotropin-releasing hormone production. WDR11 complex facilitates the tethering of Adaptor protein-1 complex (AP-1)-derived vesicles. WDR11 complex acts together with TBC1D23 to facilitate the golgin-mediated capture of vesicles generated using AP-1.
==== BP ====
GO:0006886 ! intracellular protein transport
GO:0007507 ! heart development
GO:0008589 ! regulation of smoothened signaling pathway
GO:0035264 ! multicellular organism growth
GO:0060271 ! cilium assembly
GO:0060322 ! head development
GO:0099041 ! vesicle tethering to Golgi
12 Q6ZNE9-2, RUFY4 (Chr2) regulation of protein folding
==== MF ====
GO:0003824 F 0.71 catalytic activity

==== BP ====
GO:0009987 P 0.96 cellular process
GO:0065007 P 0.78 biological regulation
GO:0071704 P 0.76 organic substance metabolic process
GO:0044238 P 0.76 primary metabolic process
GO:0044237 P 0.76 cellular metabolic process
GO:0050794 P 0.75 regulation of cellular process
GO:0006807 P 0.75 nitrogen compound metabolic process
GO:0006139 P 0.74 nucleobase-containing compound metabolic process
GO:0034654 P 0.71 nucleobase-containing compound biosynthetic process
GO:0051179 P 0.69 localization
GO:0051128 P 0.68 regulation of cellular component organization
GO:0006810 P 0.68 transport
GO:0055086 P 0.63 nucleobase-containing small molecule metabolic process
GO:0051641 P 0.63 cellular localization
GO:0006793 P 0.63 phosphorus metabolic process
GO:0046907 P 0.57 intracellular transport
GO:0031323 P 0.54 regulation of cellular metabolic process
GO:1901135 P 0.53 carbohydrate derivative metabolic process
GO:0061077 P 0.51 chaperone-mediated protein folding
GO:0006458 P 0.51 'de novo' protein folding

 0.26,0.32 Positively regulates macroautophagy in primary dendritic cells. Increases autophagic flux, probably by stimulating both autophagosome formation and facilitating tethering with lysosomes. Binds to phosphatidylinositol 3-phosphate (PtdIns3P) through its FYVE-type zinc finger.
==== MF ====
GO:0032266 ! phosphatidylinositol-3-phosphate binding

==== BP ====
GO:0000045 ! autophagosome assembly
GO:0016239 ! positive regulation of macroautophagy
GO:0071353 ! cellular response to interleukin-4
13 Q9GZU8-1, FAM192A (Chr16) hydrolase, probably hydrolase of protein
==== BP ====
GO:0009987 P 0.88 cellular process
GO:0065007 P 0.82 biological regulation
GO:0050789 P 0.80 regulation of biological process
GO:0050794 P 0.79 regulation of cellular process
GO:0008152 P 0.70 metabolic process
GO:0071704 P 0.67 organic substance metabolic process
GO:0044238 P 0.67 primary metabolic process
GO:0006807 P 0.65 nitrogen compound metabolic process
GO:0043170 P 0.61 macromolecule metabolic process
GO:1901564 P 0.55 organonitrogen compound metabolic process
GO:0071840 P 0.53 cellular component organization or biogenesis
GO:0048519 P 0.52 negative regulation of biological process
GO:0016043 P 0.52 cellular component organization
GO:0048523 P 0.51 negative regulation of cellular process
GO:0044237 P 0.51 cellular metabolic process
GO:0019538 P 0.50 protein metabolic process

 NA,0.32 Promotes the association of the proteasome activator complex subunit PSME3 with the 20S proteasome and regulates its activity. Inhibits PSME3-mediated degradation of some proteasome substrates, probably by affecting their diffusion rate into the catalytic chamber of the proteasome
==== BP ====
GO:0032091 ! negative regulation of protein binding
GO:1901799 ! negative regulation of proteasomal protein catabolic process
14 Q494U1-1, PLEKHN1 (Chr1) transmembrane transport of small molecules, such as nucleotide
==== MF ====
GO:0022857 F 0.93 transmembrane transporter activity
GO:0015075 F 0.76 ion transmembrane transporter activity
GO:0008509 F 0.75 anion transmembrane transporter activity
GO:0008514 F 0.72 organic anion transmembrane transporter activity
GO:0015605 F 0.63 organophosphate ester transmembrane transporter activity
GO:0015318 F 0.63 inorganic molecular entity transmembrane transporter activity
GO:0008028 F 0.59 monocarboxylic acid transmembrane transporter activity

==== BP ====
GO:0006810 P 0.96 transport
GO:0071702 P 0.93 organic substance transport
GO:0055085 P 0.87 transmembrane transport
GO:0009987 P 0.86 cellular process
GO:0006811 P 0.78 ion transport
GO:0034220 P 0.77 ion transmembrane transport
GO:0015711 P 0.77 organic anion transport
GO:0098656 P 0.76 anion transmembrane transport
GO:0071705 P 0.71 nitrogen compound transport
GO:1901264 P 0.68 carbohydrate derivative transport
GO:0065007 P 0.62 biological regulation
GO:0051641 P 0.57 cellular localization
GO:0050789 P 0.57 regulation of biological process
GO:0050794 P 0.56 regulation of cellular process
GO:0046907 P 0.56 intracellular transport
GO:0015931 P 0.55 nucleobase-containing compound transport
GO:0007017 P 0.55 microtubule-based process
GO:0006928 P 0.55 movement of cell or subcellular component
GO:0046903 P 0.53 secretion
GO:0030705 P 0.53 cytoskeleton-dependent intracellular transport
GO:0015780 P 0.53 nucleotide-sugar transmembrane transport

 0.05,0.29 Controls the stability of the leptin mRNA harboring an AU-rich element (ARE) in its 3' UTR, in cooperation with the RNA stabilizer ELAVL1
==== MF ====
GO:0001786 ! phosphatidylserine binding
GO:0070300 ! phosphatidic acid binding
GO:1901612 ! cardiolipin binding
GO:1901981 ! phosphatidylinositol phosphate binding

==== BP ====
GO:0001666 ! response to hypoxia
GO:0043065 ! positive regulation of apoptotic process
GO:0061158 ! 3'-UTR-mediated mRNA destabilization
15 Q8IUW5-1, RELL1 (Chr4) * regulation of apoptosis through TNF
==== BP ====
GO:0050789 P 1.00 regulation of biological process
GO:0009987 P 0.70 cellular process
GO:0050794 P 0.65 regulation of cellular process
GO:0048518 P 0.64 positive regulation of biological process
GO:0097190 P 0.51 apoptotic signaling pathway
GO:0042981 P 0.51 regulation of apoptotic process
GO:0042127 P 0.51 regulation of cell proliferation
GO:0032496 P 0.51 response to lipopolysaccharide
GO:0007275 P 0.51 multicellular organism development
GO:0006955 P 0.51 immune response
GO:0006954 P 0.51 inflammatory response

 NA,0.29 Induces activation of MAPK14/p38 cascade, when overexpressed
==== BP ====
GO:1900745 ! positive regulation of p38MAPK cascade
16 Q8NDM7-1, CFAP43 (Chr10)
==== BP ====
GO:0009987 P 0.98 cellular process
GO:0065007 P 0.70 biological regulation
GO:0050789 P 0.68 regulation of biological process
GO:0050794 P 0.66 regulation of cellular process
GO:0071840 P 0.61 cellular component organization or biogenesis
GO:0016043 P 0.60 cellular component organization

 NA,0.29 Flagellar protein involved in sperm flagellum axoneme organization and function.
==== BP ====
GO:0007288 ! sperm axoneme assembly
17 Q8TDG2-1, ACTRT1 (ChrX) regulation of chromosome organization either though histone acetylation or binding of cytoskelton used in chromsome segregation
==== MF ====
GO:0005198 F 0.90 structural molecule activity
GO:1901363 F 0.89 heterocyclic compound binding
GO:0097159 F 0.89 organic cyclic compound binding
GO:0005200 F 0.89 structural constituent of cytoskeleton
GO:0043167 F 0.85 ion binding
GO:0035639 F 0.84 purine ribonucleoside triphosphate binding
GO:0032555 F 0.84 purine ribonucleotide binding
GO:0005524 F 0.83 ATP binding
GO:0003824 F 0.77 catalytic activity
GO:0016740 F 0.66 transferase activity
GO:0042802 F 0.64 identical protein binding
GO:0004402 F 0.61 histone acetyltransferase activity
GO:0008092 F 0.59 cytoskeletal protein binding
GO:0003676 F 0.51 nucleic acid binding

==== BP ====
GO:0065007 P 0.96 biological regulation
GO:0050789 P 0.95 regulation of biological process
GO:0009987 P 0.90 cellular process
GO:0060255 P 0.89 regulation of macromolecule metabolic process
GO:0048518 P 0.89 positive regulation of biological process
GO:0010468 P 0.88 regulation of gene expression
GO:0009893 P 0.85 positive regulation of metabolic process
GO:0016043 P 0.78 cellular component organization
GO:0010604 P 0.78 positive regulation of macromolecule metabolic process
GO:0010628 P 0.77 positive regulation of gene expression
GO:0032501 P 0.76 multicellular organismal process
GO:0050794 P 0.74 regulation of cellular process
GO:0090131 P 0.71 mesenchyme migration
GO:0032502 P 0.71 developmental process
GO:0044237 P 0.70 cellular metabolic process
GO:0071704 P 0.69 organic substance metabolic process
GO:0044238 P 0.69 primary metabolic process
GO:0006996 P 0.69 organelle organization
GO:0050896 P 0.68 response to stimulus
GO:0006807 P 0.68 nitrogen compound metabolic process
GO:1901564 P 0.67 organonitrogen compound metabolic process
GO:0044260 P 0.67 cellular macromolecule metabolic process
GO:0022607 P 0.66 cellular component assembly
GO:0051716 P 0.65 cellular response to stimulus
GO:0048869 P 0.65 cellular developmental process
GO:0044267 P 0.65 cellular protein metabolic process
GO:0043412 P 0.65 macromolecule modification
GO:0042221 P 0.64 response to chemical
GO:0006950 P 0.64 response to stress
GO:0006464 P 0.64 cellular protein modification process
GO:0006325 P 0.64 chromatin organization
GO:1901360 P 0.63 organic cyclic compound metabolic process
GO:0034641 P 0.63 cellular nitrogen compound metabolic process
GO:0022402 P 0.63 cell cycle process
GO:0070887 P 0.62 cellular response to chemical stimulus
GO:0033554 P 0.62 cellular response to stress
GO:0022414 P 0.62 reproductive process
GO:0006139 P 0.62 nucleobase-containing compound metabolic process
GO:0090304 P 0.61 nucleic acid metabolic process
GO:0048646 P 0.61 anatomical structure formation involved in morphogenesis
GO:0018193 P 0.61 peptidyl-amino acid modification
GO:0016570 P 0.61 histone modification
GO:0003006 P 0.61 developmental process involved in reproduction
GO:1903046 P 0.60 meiotic cell cycle process
GO:0032506 P 0.60 cytokinetic process
GO:0016573 P 0.60 histone acetylation
GO:0010927 P 0.60 cellular component assembly involved in morphogenesis
GO:0006281 P 0.60 DNA repair
GO:0034599 P 0.59 cellular response to oxidative stress
GO:0030476 P 0.59 ascospore wall assembly
GO:0001300 P 0.59 chronological cell aging
GO:0000916 P 0.59 actomyosin contractile ring contraction
GO:0000011 P 0.59 vacuole inheritance
GO:0080090 P 0.57 regulation of primary metabolic process
GO:0051171 P 0.57 regulation of nitrogen compound metabolic process
GO:0031323 P 0.57 regulation of cellular metabolic process
GO:0031326 P 0.54 regulation of cellular biosynthetic process
GO:0019219 P 0.54 regulation of nucleobase-containing compound metabolic process
GO:0010556 P 0.54 regulation of macromolecule biosynthetic process
GO:0048522 P 0.53 positive regulation of cellular process
GO:0048519 P 0.53 negative regulation of biological process
GO:0006355 P 0.53 regulation of transcription, DNA-templated
GO:0051179 P 0.51 localization
GO:0048523 P 0.51 negative regulation of cellular process

 0.03,0.29 Negatively regulates the Hedgehog (SHH) signaling. Binds to the promoter of the SHH signaling mediator, GLI1, and inhibits its expression.
==== MF ====
GO:0003682 ! chromatin binding

==== BP ====
GO:0008589 ! regulation of smoothened signaling pathway
GO:0045892 ! negative regulation of transcription, DNA-templated
18 O75677-1, RFPL1 (Chr22) * ubiquitin-protein transferase activity
==== BP ====
GO:0009987 P 0.97 cellular process
GO:0065007 P 0.93 biological regulation
GO:0050789 P 0.92 regulation of biological process
GO:0050794 P 0.88 regulation of cellular process
GO:0008152 P 0.83 metabolic process
GO:0044237 P 0.82 cellular metabolic process
GO:0071704 P 0.75 organic substance metabolic process
GO:0044238 P 0.74 primary metabolic process
GO:0006807 P 0.72 nitrogen compound metabolic process
GO:0048583 P 0.71 regulation of response to stimulus
GO:0043170 P 0.70 macromolecule metabolic process
GO:0048519 P 0.69 negative regulation of biological process
GO:0048518 P 0.69 positive regulation of biological process
GO:0019222 P 0.69 regulation of metabolic process
GO:0050896 P 0.68 response to stimulus
GO:0016043 P 0.68 cellular component organization
GO:1901564 P 0.67 organonitrogen compound metabolic process
GO:0031323 P 0.67 regulation of cellular metabolic process
GO:0060255 P 0.66 regulation of macromolecule metabolic process
GO:0044260 P 0.66 cellular macromolecule metabolic process
GO:0080090 P 0.65 regulation of primary metabolic process
GO:0051171 P 0.65 regulation of nitrogen compound metabolic process
GO:0023051 P 0.65 regulation of signaling
GO:0019538 P 0.65 protein metabolic process
GO:0010646 P 0.65 regulation of cell communication
GO:0009966 P 0.64 regulation of signal transduction
GO:0002376 P 0.63 immune system process
GO:0048523 P 0.61 negative regulation of cellular process
GO:0006950 P 0.61 response to stress
GO:0048522 P 0.59 positive regulation of cellular process
GO:0044267 P 0.59 cellular protein metabolic process
GO:0043412 P 0.59 macromolecule modification
GO:0006464 P 0.58 cellular protein modification process
GO:0010468 P 0.56 regulation of gene expression
GO:0016567 P 0.55 protein ubiquitination
GO:0051252 P 0.54 regulation of RNA metabolic process
GO:0031326 P 0.54 regulation of cellular biosynthetic process
GO:0006355 P 0.53 regulation of transcription, DNA-templated
GO:0006952 P 0.51 defense response

 NA,0.27 Negatively regulates the G2-M phase transition, possibly by promoting cyclin B1/CCNB1 and CDK1 proteasomal degradation and thereby preventing their accumulation during interphase.
==== BP ====
GO:0007049 ! cell cycle
GO:0008285 ! negative regulation of cell proliferation
GO:0010972 ! negative regulation of G2/M transition of mitotic cell cycle
GO:0032436 ! positive regulation of proteasomal ubiquitin-dependent protein catabolic process
GO:0043065 ! positive regulation of apoptotic process
GO:0045930 ! negative regulation of mitotic cell cycle
GO:0051782 ! negative regulation of cell division
GO:2001272 ! positive regulation of cysteine-type endopeptidase activity involved in execution phase of apoptosis
19 Q5VTQ0-1, TTC39B (Chr9) * protein ubiquitination regulation
==== BP ====
GO:0009987 P 0.96 cellular process
GO:0065007 P 0.81 biological regulation
GO:0050789 P 0.79 regulation of biological process
GO:0050794 P 0.76 regulation of cellular process
GO:0008152 P 0.73 metabolic process
GO:0071704 P 0.72 organic substance metabolic process
GO:0044237 P 0.72 cellular metabolic process
GO:0044238 P 0.71 primary metabolic process

 NA,0.26 Regulates high density lipoprotein (HDL) cholesterol metabolism by promoting the ubiquitination and degradation of the oxysterols receptors LXR (NR1H2 and NR1H3).
==== BP ====
GO:0006629 ! lipid metabolic process
GO:0010874 ! regulation of cholesterol efflux
GO:0010887 ! negative regulation of cholesterol storage
GO:0042632 ! cholesterol homeostasis
GO:0090181 ! regulation of cholesterol metabolic process
20 Q96S16-1, JMJD8 (Chr16) histone demethylation
==== BP ====
GO:0009987 P 0.99 cellular process
GO:0071704 P 0.97 organic substance metabolic process
GO:0044238 P 0.97 primary metabolic process
GO:0044237 P 0.97 cellular metabolic process
GO:1901564 P 0.96 organonitrogen compound metabolic process
GO:0043170 P 0.96 macromolecule metabolic process
GO:0043412 P 0.95 macromolecule modification
GO:0044260 P 0.92 cellular macromolecule metabolic process
GO:0019538 P 0.89 protein metabolic process
GO:0006464 P 0.88 cellular protein modification process
GO:0050789 P 0.84 regulation of biological process
GO:0050794 P 0.77 regulation of cellular process
GO:0019222 P 0.76 regulation of metabolic process
GO:0080090 P 0.75 regulation of primary metabolic process
GO:0060255 P 0.75 regulation of macromolecule metabolic process
GO:0031323 P 0.75 regulation of cellular metabolic process
GO:0051171 P 0.74 regulation of nitrogen compound metabolic process
GO:0019219 P 0.71 regulation of nucleobase-containing compound metabolic process
GO:0010468 P 0.71 regulation of gene expression
GO:0051252 P 0.70 regulation of RNA metabolic process
GO:0016043 P 0.67 cellular component organization
GO:0018193 P 0.63 peptidyl-amino acid modification
GO:0006325 P 0.63 chromatin organization
GO:0016570 P 0.61 histone modification
GO:0016577 P 0.52 histone demethylation
GO:0031326 P 0.50 regulation of cellular biosynthetic process

 NA,0.21 Functions as a positive regulator of TNF-induced NF-kappa-B signaling. Regulates angiogenesis and cellular metabolism through interaction with PKM.
==== BP ====
GO:0006110 ! regulation of glycolytic process
GO:0043123 ! positive regulation of I-kappaB kinase/NF-kappaB signaling
GO:1903302 ! regulation of pyruvate kinase activity
GO:1903672 ! positive regulation of sprouting angiogenesis
21 Q9H9L7-1, AKIRIN1 (Chr1) by binding to RNA polymerase, regulate expression of genes such as cytokines
==== BP ====
GO:0045944 P 1.00 positive regulation of transcription by RNA polymerase II
GO:0050896 P 0.91 response to stimulus
GO:0009605 P 0.90 response to external stimulus
GO:0043207 P 0.89 response to external biotic stimulus
GO:0009987 P 0.85 cellular process
GO:1901700 P 0.71 response to oxygen-containing compound
GO:0051239 P 0.71 regulation of multicellular organismal process
GO:0010033 P 0.71 response to organic substance
GO:0051240 P 0.70 positive regulation of multicellular organismal process
GO:0033993 P 0.69 response to lipid
GO:0032496 P 0.68 response to lipopolysaccharide
GO:0001819 P 0.68 positive regulation of cytokine production
GO:0032755 P 0.67 positive regulation of interleukin-6 production
GO:0032502 P 0.65 developmental process
GO:0048856 P 0.61 anatomical structure development

 NA,0.18 Functions as signal transducer for MSTN during skeletal muscle regeneration and myogenesis. May regulates chemotaxis of both macrophages and myoblasts by reorganising actin cytoskeleton, leading to more efficient lamellipodia formation via a PI3 kinase dependent pathway.
==== BP ====
GO:0010592 ! positive regulation of lamellipodium assembly
GO:0010759 ! positive regulation of macrophage chemotaxis
GO:0014839 ! myoblast migration involved in skeletal muscle regeneration
GO:0045663 ! positive regulation of myoblast differentiation
GO:1902723 ! negative regulation of skeletal muscle satellite cell proliferation
GO:1902725 ! negative regulation of satellite cell differentiation
22 Q96KV7-1, WDR90 (Chr16) regulation of transcription by nucleic acid binding
==== BP ====
GO:0009987 P 0.92 cellular process
GO:0065007 P 0.84 biological regulation
GO:0050789 P 0.82 regulation of biological process
GO:0050794 P 0.80 regulation of cellular process
GO:0019222 P 0.66 regulation of metabolic process
GO:0060255 P 0.64 regulation of macromolecule metabolic process
GO:0080090 P 0.63 regulation of primary metabolic process
GO:0031323 P 0.63 regulation of cellular metabolic process
GO:0051171 P 0.62 regulation of nitrogen compound metabolic process
GO:0010468 P 0.60 regulation of gene expression
GO:0019219 P 0.59 regulation of nucleobase-containing compound metabolic process
GO:2000112 P 0.58 regulation of cellular macromolecule biosynthetic process
GO:0051252 P 0.58 regulation of RNA metabolic process
GO:0071840 P 0.57 cellular component organization or biogenesis
GO:0006355 P 0.57 regulation of transcription, DNA-templated
GO:0044237 P 0.56 cellular metabolic process
GO:0016043 P 0.54 cellular component organization

 NA,0.17 Required for efficient primary cilium formation.
==== BP ====
GO:0060271 ! cilium assembly
23 Q6AI39-1, BICRAL (Chr6) sodium:potassium ion transporter
 NA,NA Component of SWI/SNF chromatin remodeling subcomplex GBAF that carries out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner.
24 Q96J88-1, EPSTI1 (Chr13) cytoskeleton binding
 NA,NA Plays a role in M1 macrophage polarization and is required for the proper regulation of gene expression during M1 versus M2 macrophage differentiation. Might play a role in RELA/p65 and STAT1 phosphorylation and nuclear localization upon activation of macrophages.
25 Q9BZD6-1, PRRG4 (Chr11) serine-type endopeptidase
 NA,NA May control axon guidance across the CNS. Prevents the delivery of ROBO1 at the cell surface and downregulates its expression.


2. Methods and overall results for 44 neXtProt proteins

This section summarizes GO term predictions by I-TASSER/COFACTOR for the canonical isoforms of 44 neXtProt targets undergoing literature curation by neXtProt as of 2018.

GO terms considered by neXtProt as too general (https://www.nextprot.org/proteins/search?mode=advanced&queryId=NXQ_00022) are excluded. For each target, we list predicted GO terms in three blocks corresponding to the three aspects of GO: Molecular Function (MF), Biological Process (BP), and Cellular Component (CC). All GO terms within an aspect are ranked in descending order of confidence score (C-score). C-score ranges between 0 and 1. GO terms with <0.50 C-score values are usually excluded. However, we are presently holding a small number of terms with scores ≥ 0.40 (in grey) that might be useful for proposing the function.

Based on the predicted GO terms, we manually assign a free-text function annotation following each target. This results in 38 out of 44 (86%) of the targets having free-text manual function interpretations by their accession. Among the 38 function interpretations, 3 of them are relatively generic enzymatic functions that are not excluded by neXtProt: 
Q6ZW76-1	transferase acting on a protein
Q96D15-1	catalytic activity, acting on a protein
Q96II8-1	transferase acting on a protein
The remaining interpretations for 35 out of all 44 targets (80%) are reasonably specific. These specific functions range from ubiquitin-protein transferase (A6NLU0-1, O75677-1) and phosphatidylinositol-4-phosphate phosphatase (Q5T0D9-1) to heat shock protein binding (O95801-1) and structural component of eye lens (Q8WXF5-1).

In this study, 27 (61%) and 43 (98%) out of 44 targets acquire specific MF and BP prediction with C-score≥0.5, respectively, while in our 2018 study (DOI: 10.1021/acs.jproteome.8b00453), only 13 (20%) and 33 (50%) out of 66 (20%) uPE1 proteins receive confident MF predictions. Such differences may be explained by the nature of the two datasets: our earlier study in 2018 focus on the complete set of 66 uPE1 Chr17 proteins not known to be studied previously. Here, we test our methods blindly on the 44 proteins chosen by neXtProt curators for their literature-based function annotations in preparation for the next neXtProt release in 2019.

We note that some predicted GO terms have >0.9 or even 1.00 C-score, especially for more general terms. This resulted from the design of C-score system used by our COFACTOR function annotation, and reflects the hierarchical nature of GO term where parent terms are more confidently predicted than specific child terms. In COFACTOR, the C-score for GO term q is determined by: where m∈{sequence,structure,PPI} are the three component methods of COFACTOR. The C-score for q by the mth method is determined by: where Sim is the confidence score of ith template used by method m. The template confidence could be BLAST/PSI-BLAST sequence identity for sequence based method, and interaction score assigned by STRING database for PPI based method. Sim(q) is the confidence of ith template in method m for the subset of templates associated with GO term q. Therefore, if all templates are all associated with q, the nominator and denominator in equation (4) are the same, and Cscore(q) is 1.00 even when none of the templates shares high sequence similarity to query.

As a technical note, we note that the sequence-based component method uses (PSI-)BLAST hits against UniProt database as sequence templates. All predictions presented here uses a relatively old UniProt database version 2018_02, while predictions using a more recent UniProt database 2018_11 is separately available here. The reason for using an older version of template database is explained as a technical note at this page.

Table 3. Predicted GO terms by I-TASSER/COFACTOR for all 44 neXtProt proteins undergoing literature curation as of 2018.

The targets are ranked in alphabetical order of the accession and separated by four-dollar ($$) signs.
Information identical to this table is also provided in a zip file to facilitate programmatic parsing
of the findings (GOterm.zip).


A6NI56-1	transferase acting on a protein
==== MF ====
*	GO:0140096	0.50	catalytic activity, acting on a protein
*	GO:0016740	0.48	transferase activity
==== BP ====
	GO:0009987	0.98	cellular process
	GO:0065007	0.81	biological regulation
	GO:0050789	0.78	regulation of biological process
	GO:0050794	0.77	regulation of cellular process
	GO:0051179	0.70	localization
	GO:0071840	0.68	cellular component organization or biogenesis
	GO:0016043	0.67	cellular component organization
	GO:0008152	0.63	metabolic process
	GO:0044237	0.61	cellular metabolic process
	GO:0048518	0.59	positive regulation of biological process
	GO:0071704	0.58	organic substance metabolic process
	GO:0048522	0.58	positive regulation of cellular process
	GO:0044238	0.57	primary metabolic process
	GO:0051128	0.56	regulation of cellular component organization
	GO:0043170	0.56	macromolecule metabolic process
	GO:0006807	0.56	nitrogen compound metabolic process
	GO:1901564	0.52	organonitrogen compound metabolic process
	GO:0044260	0.51	cellular macromolecule metabolic process
	GO:0022402	0.51	cell cycle process
	GO:0019538	0.51	protein metabolic process
==== CC ====
	GO:0044424	1.00	intracellular part
	GO:0043226	0.89	organelle
	GO:0044446	0.87	intracellular organelle part
	GO:0044444	0.87	cytoplasmic part
	GO:0043227	0.83	membrane-bounded organelle
	GO:0043229	0.81	intracellular organelle
	GO:0032991	0.75	protein-containing complex
	GO:0043231	0.71	intracellular membrane-bounded organelle
	GO:0005737	0.52	cytoplasm
	GO:0044430	0.51	cytoskeletal part
$$$$

A6NLU0-1	ubiquitin-protein transferase
==== MF ====
	GO:0016740	0.83	transferase activity
	GO:0140096	0.82	catalytic activity, acting on a protein
*	GO:0004842	0.80	ubiquitin-protein transferase activity
==== BP ====
	GO:0009987	0.96	cellular process
	GO:0050789	0.94	regulation of biological process
	GO:0050794	0.90	regulation of cellular process
	GO:0008152	0.80	metabolic process
	GO:0044237	0.78	cellular metabolic process
	GO:0048583	0.76	regulation of response to stimulus
	GO:0048519	0.75	negative regulation of biological process
	GO:0071704	0.72	organic substance metabolic process
	GO:0050896	0.71	response to stimulus
	GO:0044238	0.71	primary metabolic process
	GO:0023051	0.71	regulation of signaling
	GO:0016043	0.71	cellular component organization
	GO:0010646	0.71	regulation of cell communication
	GO:0019222	0.70	regulation of metabolic process
	GO:0009966	0.70	regulation of signal transduction
	GO:0048518	0.69	positive regulation of biological process
	GO:0006807	0.69	nitrogen compound metabolic process
	GO:0048523	0.68	negative regulation of cellular process
	GO:0043170	0.68	macromolecule metabolic process
	GO:0031323	0.67	regulation of cellular metabolic process
	GO:0080090	0.65	regulation of primary metabolic process
	GO:0060255	0.65	regulation of macromolecule metabolic process
	GO:0044260	0.65	cellular macromolecule metabolic process
	GO:0006950	0.65	response to stress
	GO:0051171	0.64	regulation of nitrogen compound metabolic process
	GO:1901564	0.63	organonitrogen compound metabolic process
	GO:0019538	0.61	protein metabolic process
	GO:0048522	0.59	positive regulation of cellular process
	GO:0043412	0.57	macromolecule modification
	GO:0006464	0.56	cellular protein modification process
	GO:0010468	0.55	regulation of gene expression
	GO:0002376	0.55	immune system process
	GO:0019219	0.54	regulation of nucleobase-containing compound metabolic process
	GO:2000112	0.53	regulation of cellular macromolecule biosynthetic process
	GO:0051252	0.53	regulation of RNA metabolic process
	GO:0048585	0.52	negative regulation of response to stimulus
	GO:0007166	0.52	cell surface receptor signaling pathway
*	GO:0016567	0.51	protein ubiquitination
	GO:0006355	0.51	regulation of transcription, DNA-templated
	GO:0009968	0.50	negative regulation of signal transduction
==== CC ====
	GO:0044464	1.00	cell part
	GO:0044424	0.99	intracellular part
	GO:0043226	0.83	organelle
	GO:0043229	0.80	intracellular organelle
	GO:0044444	0.73	cytoplasmic part
	GO:0043227	0.71	membrane-bounded organelle
	GO:0043231	0.67	intracellular membrane-bounded organelle
	GO:0005737	0.62	cytoplasm
	GO:0044422	0.61	organelle part
	GO:0044446	0.60	intracellular organelle part
	GO:0032991	0.60	protein-containing complex
	GO:0005634	0.55	nucleus
$$$$

A8MYV0-1	Protein serine/threonine kinase acting on microtubule
==== MF ====
*	GO:0004674	0.69	protein serine/threonine kinase activity
==== BP ====
	GO:0009987	0.99	cellular process
	GO:0032502	0.73	developmental process
	GO:0016043	0.71	cellular component organization
	GO:0019538	0.67	protein metabolic process
	GO:0065007	0.61	biological regulation
	GO:0048869	0.61	cellular developmental process
	GO:0050789	0.60	regulation of biological process
	GO:0050794	0.59	regulation of cellular process
	GO:0048856	0.59	anatomical structure development
	GO:0044237	0.54	cellular metabolic process
	GO:0022607	0.54	cellular component assembly
	GO:0006996	0.54	organelle organization
*	GO:0006468	0.53	protein phosphorylation
	GO:0050896	0.48	response to stimulus
*	GO:0007017	0.48	microtubule-based process
*	GO:0000226	0.47	microtubule cytoskeleton organization
==== CC ====
	GO:0044464	1.00	cell part
	GO:0044424	0.96	intracellular part
	GO:0043226	0.74	organelle
	GO:0044444	0.57	cytoplasmic part
	GO:0043229	0.52	intracellular organelle
*	GO:0005856	0.47	cytoskeleton
$$$$

O14894-1	
==== MF ====
==== BP ====
	GO:0050789	0.89	regulation of biological process
	GO:0050794	0.87	regulation of cellular process
	GO:0009987	0.86	cellular process
	GO:0032502	0.79	developmental process
	GO:0048856	0.76	anatomical structure development
	GO:0048519	0.67	negative regulation of biological process
	GO:0048523	0.66	negative regulation of cellular process
	GO:0048869	0.62	cellular developmental process
	GO:0019222	0.52	regulation of metabolic process
	GO:0060255	0.50	regulation of macromolecule metabolic process
	GO:0031323	0.50	regulation of cellular metabolic process
==== CC ====
	GO:0016021	1.00	integral component of membrane
	GO:0044464	0.97	cell part
	GO:0044424	0.71	intracellular part
	GO:0044459	0.58	plasma membrane part
	GO:0005887	0.53	integral component of plasma membrane
$$$$

O60829-1	hydratase (hydrolyase) to degrade phosphate containing molecules, such as RNA
==== MF ====
==== BP ====
*	GO:0006139	0.50	nucleobase-containing compound metabolic process
==== CC ====
*	GO:0000015	1.00	phosphopyruvate hydratase complex
	GO:0005737	0.94	cytoplasm
	GO:0043226	0.65	organelle
	GO:1990904	0.64	ribonucleoprotein complex
	GO:0043229	0.58	intracellular organelle
	GO:0009986	0.56	cell surface
	GO:0043232	0.53	intracellular non-membrane-bounded organelle
*	GO:0043186	0.50	P granule
$$$$

O60941-1	cytoskeleton-associated protein, possibly regulating muscle contraction
==== MF ====
*	GO:0008092	0.79	cytoskeletal protein binding
*	GO:0005198	0.54	structural molecule activity
*	GO:0008307	0.49	structural constituent of muscle
==== BP ====
	GO:0009987	0.97	cellular process
	GO:0065007	0.81	biological regulation
	GO:0050789	0.79	regulation of biological process
	GO:0016043	0.78	cellular component organization
	GO:0032502	0.74	developmental process
	GO:0048856	0.65	anatomical structure development
	GO:0050794	0.63	regulation of cellular process
	GO:0032501	0.62	multicellular organismal process
	GO:0006996	0.57	organelle organization
*	GO:0061061	0.55	muscle structure development
	GO:0048518	0.54	positive regulation of biological process
*	GO:0007010	0.49	cytoskeleton organization
	GO:0048513	0.47	animal organ development
	GO:0003008	0.47	system process
*	GO:0030029	0.46	actin filament-based process
==== CC ====
	GO:0044424	1.00	intracellular part
	GO:0044425	0.92	membrane part
	GO:0044459	0.90	plasma membrane part
	GO:0032991	0.90	protein-containing complex
	GO:0098797	0.82	plasma membrane protein complex
*	GO:0016010	0.75	dystrophin-associated glycoprotein complex
	GO:0016020	0.69	membrane
	GO:0044422	0.68	organelle part
	GO:0044444	0.67	cytoplasmic part
	GO:0044446	0.63	intracellular organelle part
	GO:0043226	0.63	organelle
	GO:0005886	0.63	plasma membrane
	GO:0043229	0.62	intracellular organelle
	GO:0005737	0.53	cytoplasm
	GO:0043227	0.51	membrane-bounded organelle
*	GO:0042383	0.49	sarcolemma
$$$$

O75363-1	(for CC: neuron part)
==== MF ====
	GO:0042803	0.63	protein homodimerization activity
==== BP ====
	GO:0009987	0.90	cellular process
	GO:0065007	0.81	biological regulation
	GO:0050789	0.74	regulation of biological process
	GO:0050794	0.72	regulation of cellular process
	GO:0008152	0.70	metabolic process
==== CC ====
	GO:0044464	1.00	cell part
	GO:0044424	0.92	intracellular part
	GO:0043226	0.74	organelle
	GO:0043227	0.70	membrane-bounded organelle
	GO:0044444	0.58	cytoplasmic part
	GO:0043229	0.52	intracellular organelle
	GO:0044421	0.47	extracellular region part
	GO:0043231	0.46	intracellular membrane-bounded organelle
	GO:0044422	0.43	organelle part
*	GO:0097458	0.42	neuron part
$$$$

O75677-1	ubiquitin-protein transferase activity
==== MF ====
	GO:0140096	0.80	catalytic activity, acting on a protein
	GO:0016740	0.80	transferase activity
*	GO:0004842	0.78	ubiquitin-protein transferase activity
==== BP ====
	GO:0009987	0.97	cellular process
	GO:0065007	0.93	biological regulation
	GO:0050789	0.92	regulation of biological process
	GO:0050794	0.88	regulation of cellular process
	GO:0008152	0.83	metabolic process
	GO:0044237	0.82	cellular metabolic process
	GO:0071704	0.75	organic substance metabolic process
	GO:0044238	0.74	primary metabolic process
	GO:0006807	0.72	nitrogen compound metabolic process
	GO:0048583	0.71	regulation of response to stimulus
	GO:0043170	0.70	macromolecule metabolic process
	GO:0048519	0.69	negative regulation of biological process
	GO:0048518	0.69	positive regulation of biological process
	GO:0019222	0.69	regulation of metabolic process
	GO:0050896	0.68	response to stimulus
	GO:0016043	0.68	cellular component organization
	GO:1901564	0.67	organonitrogen compound metabolic process
	GO:0031323	0.67	regulation of cellular metabolic process
	GO:0060255	0.66	regulation of macromolecule metabolic process
	GO:0044260	0.66	cellular macromolecule metabolic process
	GO:0080090	0.65	regulation of primary metabolic process
	GO:0051171	0.65	regulation of nitrogen compound metabolic process
	GO:0023051	0.65	regulation of signaling
	GO:0019538	0.65	protein metabolic process
	GO:0010646	0.65	regulation of cell communication
	GO:0009966	0.64	regulation of signal transduction
	GO:0002376	0.63	immune system process
	GO:0048523	0.61	negative regulation of cellular process
	GO:0006950	0.61	response to stress
	GO:0048522	0.59	positive regulation of cellular process
	GO:0044267	0.59	cellular protein metabolic process
	GO:0043412	0.59	macromolecule modification
	GO:0006464	0.58	cellular protein modification process
	GO:0010468	0.56	regulation of gene expression
*	GO:0016567	0.55	protein ubiquitination
	GO:0051252	0.54	regulation of RNA metabolic process
	GO:0031326	0.54	regulation of cellular biosynthetic process
	GO:0006355	0.53	regulation of transcription, DNA-templated
	GO:0006952	0.51	defense response
==== CC ====
	GO:0044464	1.00	cell part
	GO:0044424	0.99	intracellular part
	GO:0043226	0.81	organelle
	GO:0043229	0.79	intracellular organelle
	GO:0044444	0.73	cytoplasmic part
	GO:0043227	0.68	membrane-bounded organelle
	GO:0032991	0.66	protein-containing complex
	GO:0043231	0.64	intracellular membrane-bounded organelle
	GO:0044422	0.63	organelle part
	GO:0044446	0.62	intracellular organelle part
	GO:0005737	0.59	cytoplasm
	GO:0005634	0.54	nucleus
$$$$

O95801-1	heat shock protein binding
==== MF ====
	GO:0140096	0.59	catalytic activity, acting on a protein
*	GO:0031072	0.48	heat shock protein binding
==== BP ====
	GO:0009987	0.96	cellular process
	GO:0065007	0.68	biological regulation
	GO:0050789	0.65	regulation of biological process
	GO:0008152	0.64	metabolic process
	GO:0071704	0.63	organic substance metabolic process
	GO:0044237	0.63	cellular metabolic process
	GO:0044238	0.62	primary metabolic process
	GO:0050794	0.59	regulation of cellular process
	GO:0071840	0.56	cellular component organization or biogenesis
	GO:0016043	0.55	cellular component organization
	GO:0006807	0.52	nitrogen compound metabolic process
	GO:0043170	0.50	macromolecule metabolic process
==== CC ====
	GO:0044424	1.00	intracellular part
	GO:0044444	0.93	cytoplasmic part
	GO:0005737	0.84	cytoplasm
	GO:0043226	0.79	organelle
	GO:0043229	0.77	intracellular organelle
	GO:0016020	0.74	membrane
	GO:0043227	0.73	membrane-bounded organelle
	GO:0044422	0.72	organelle part
	GO:0044446	0.71	intracellular organelle part
	GO:0043231	0.69	intracellular membrane-bounded organelle
	GO:0005829	0.66	cytosol
	GO:0032991	0.64	protein-containing complex
	GO:0005634	0.59	nucleus
	GO:0005886	0.56	plasma membrane
	GO:0044428	0.51	nuclear part
$$$$

P0C870-1	histone demethylation
==== MF ====
	GO:0016491	0.79	oxidoreductase activity
	GO:0016705	0.78	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
	GO:0140096	0.77	catalytic activity, acting on a protein
*	GO:0016706	0.76	2-oxoglutarate-dependent dioxygenase activity
	GO:1901363	0.69	heterocyclic compound binding
	GO:0097159	0.69	organic cyclic compound binding
*	GO:0032452	0.63	histone demethylase activity
	GO:0043167	0.56	ion binding
*	GO:0003682	0.51	chromatin binding
==== BP ====
	GO:0009987	1.00	cellular process
	GO:0008152	0.98	metabolic process
	GO:0071704	0.97	organic substance metabolic process
	GO:0044238	0.97	primary metabolic process
	GO:0044237	0.97	cellular metabolic process
	GO:1901564	0.96	organonitrogen compound metabolic process
	GO:0043412	0.95	macromolecule modification
	GO:0044260	0.84	cellular macromolecule metabolic process
	GO:0065007	0.78	biological regulation
	GO:0050789	0.77	regulation of biological process
	GO:0006464	0.76	cellular protein modification process
	GO:0050794	0.71	regulation of cellular process
	GO:0019222	0.64	regulation of metabolic process
	GO:0060255	0.63	regulation of macromolecule metabolic process
	GO:0080090	0.62	regulation of primary metabolic process
	GO:0051171	0.62	regulation of nitrogen compound metabolic process
	GO:0031323	0.62	regulation of cellular metabolic process
	GO:1901576	0.61	organic substance biosynthetic process
	GO:0016043	0.60	cellular component organization
	GO:0019219	0.59	regulation of nucleobase-containing compound metabolic process
	GO:0010468	0.59	regulation of gene expression
	GO:0051252	0.58	regulation of RNA metabolic process
	GO:1901360	0.56	organic cyclic compound metabolic process
	GO:0051179	0.56	localization
	GO:0046483	0.56	heterocycle metabolic process
	GO:0006725	0.56	cellular aromatic compound metabolic process
	GO:1901137	0.55	carbohydrate derivative biosynthetic process
	GO:0050896	0.55	response to stimulus
	GO:0006810	0.55	transport
	GO:0006139	0.55	nucleobase-containing compound metabolic process
	GO:0031590	0.54	wybutosine metabolic process
	GO:0009451	0.54	RNA modification
	GO:0008033	0.54	tRNA processing
*	GO:0016570	0.53	histone modification
	GO:0051716	0.52	cellular response to stimulus
	GO:0042221	0.50	response to chemical
==== CC ====
	GO:0044424	1.00	intracellular part
	GO:0043229	0.89	intracellular organelle
	GO:0043227	0.88	membrane-bounded organelle
	GO:0043231	0.87	intracellular membrane-bounded organelle
	GO:0005634	0.77	nucleus
	GO:0044446	0.72	intracellular organelle part
	GO:0044444	0.65	cytoplasmic part
	GO:0032991	0.63	protein-containing complex
	GO:0042597	0.61	periplasmic space
	GO:1902494	0.60	catalytic complex
	GO:0044428	0.57	nuclear part
$$$$

P0DPB3-1	DNA binding
==== MF ====
	GO:1901363	0.61	heterocyclic compound binding
	GO:0097159	0.61	organic cyclic compound binding
*	GO:0003677	0.48	DNA binding
==== BP ====
	GO:0009987	1.00	cellular process
	GO:0050789	0.97	regulation of biological process
	GO:0050794	0.93	regulation of cellular process
	GO:0048518	0.72	positive regulation of biological process
	GO:0048522	0.71	positive regulation of cellular process
	GO:0032502	0.64	developmental process
	GO:0016043	0.63	cellular component organization
	GO:0060255	0.62	regulation of macromolecule metabolic process
	GO:0051128	0.61	regulation of cellular component organization
	GO:0050793	0.59	regulation of developmental process
	GO:0009893	0.59	positive regulation of metabolic process
	GO:0010604	0.58	positive regulation of macromolecule metabolic process
	GO:0080090	0.57	regulation of primary metabolic process
	GO:0031323	0.57	regulation of cellular metabolic process
	GO:0051171	0.56	regulation of nitrogen compound metabolic process
	GO:0051173	0.53	positive regulation of nitrogen compound metabolic process
	GO:0031325	0.53	positive regulation of cellular metabolic process
	GO:0008152	0.53	metabolic process
	GO:0071704	0.52	organic substance metabolic process
==== CC ====
	GO:0044464	1.00	cell part
	GO:0044424	0.96	intracellular part
	GO:0005737	0.74	cytoplasm
	GO:0044444	0.59	cytoplasmic part
	GO:0044422	0.58	organelle part
	GO:0016020	0.57	membrane
	GO:0043226	0.54	organelle
	GO:0043229	0.51	intracellular organelle
	GO:0030054	0.50	cell junction
$$$$

P56378-1	ATPase or ATP synthase in mitochondrion
==== MF ====
	GO:0016787	0.55	hydrolase activity
*	GO:0016887	0.49	ATPase activity
==== BP ====
	GO:0009987	0.83	cellular process
	GO:0008152	0.72	metabolic process
	GO:0044237	0.71	cellular metabolic process
	GO:0071704	0.68	organic substance metabolic process
	GO:0044238	0.68	primary metabolic process
	GO:0006807	0.67	nitrogen compound metabolic process
	GO:0034641	0.60	cellular nitrogen compound metabolic process
	GO:1901360	0.59	organic cyclic compound metabolic process
	GO:0006139	0.58	nucleobase-containing compound metabolic process
	GO:1901564	0.55	organonitrogen compound metabolic process
	GO:0006810	0.54	transport
==== CC ====
	GO:0005753	1.00	mitochondrial proton-transporting ATP synthase complex
	GO:0043229	0.80	intracellular organelle
	GO:0043227	0.78	membrane-bounded organelle
	GO:0043231	0.77	intracellular membrane-bounded organelle
*	GO:0005739	0.70	mitochondrion
$$$$

P60827-1	signaling receptor binding
==== MF ====
	GO:0005102	0.41	signaling receptor binding
==== BP ====
	GO:0009987	0.93	cellular process
	GO:0065007	0.85	biological regulation
	GO:0050789	0.82	regulation of biological process
	GO:0048518	0.67	positive regulation of biological process
	GO:0032502	0.65	developmental process
	GO:0050896	0.62	response to stimulus
	GO:0050794	0.62	regulation of cellular process
	GO:0048583	0.62	regulation of response to stimulus
	GO:0016043	0.61	cellular component organization
	GO:0008152	0.61	metabolic process
	GO:0071704	0.56	organic substance metabolic process
	GO:0044238	0.54	primary metabolic process
	GO:0048584	0.53	positive regulation of response to stimulus
==== CC ====
	GO:0016020	1.00	membrane
	GO:0044464	0.83	cell part
	GO:0044421	0.77	extracellular region part
	GO:0044424	0.65	intracellular part
	GO:0005615	0.58	extracellular space
	GO:0043226	0.53	organelle
	GO:0043227	0.52	membrane-bounded organelle
	GO:0005576	0.50	extracellular region
$$$$

P84157-1	
==== MF ====
==== BP ====
	GO:0009987	0.88	cellular process
	GO:0065007	0.71	biological regulation
	GO:0050789	0.66	regulation of biological process
	GO:0008152	0.64	metabolic process
	GO:0050794	0.61	regulation of cellular process
	GO:0044237	0.61	cellular metabolic process
	GO:0071704	0.50	organic substance metabolic process
==== CC ====
	GO:0044424	1.00	intracellular part
	GO:0044446	0.84	intracellular organelle part
	GO:0032991	0.82	protein-containing complex
	GO:0043226	0.77	organelle
	GO:0043229	0.75	intracellular organelle
	GO:0043227	0.73	membrane-bounded organelle
	GO:0043231	0.70	intracellular membrane-bounded organelle
	GO:0005634	0.61	nucleus
	GO:0044444	0.56	cytoplasmic part
	GO:0005737	0.53	cytoplasm
	GO:0044428	0.51	nuclear part
$$$$

Q494U1-1	transmembrane transport of small molecules, such as nucleotide
==== MF ====
	GO:0022857	0.93	transmembrane transporter activity
	GO:0015075	0.76	ion transmembrane transporter activity
	GO:0008509	0.75	anion transmembrane transporter activity
	GO:0008514	0.72	organic anion transmembrane transporter activity
	GO:0015605	0.63	organophosphate ester transmembrane transporter activity
	GO:0015318	0.63	inorganic molecular entity transmembrane transporter activity
*	GO:0008028	0.59	monocarboxylic acid transmembrane transporter activity
==== BP ====
	GO:0006810	0.96	transport
	GO:0071702	0.93	organic substance transport
	GO:0055085	0.87	transmembrane transport
	GO:0009987	0.86	cellular process
	GO:0006811	0.78	ion transport
	GO:0034220	0.77	ion transmembrane transport
	GO:0015711	0.77	organic anion transport
	GO:0098656	0.76	anion transmembrane transport
	GO:0071705	0.71	nitrogen compound transport
	GO:1901264	0.68	carbohydrate derivative transport
	GO:0065007	0.62	biological regulation
	GO:0051641	0.57	cellular localization
	GO:0050789	0.57	regulation of biological process
	GO:0050794	0.56	regulation of cellular process
	GO:0046907	0.56	intracellular transport
	GO:0015931	0.55	nucleobase-containing compound transport
	GO:0007017	0.55	microtubule-based process
	GO:0006928	0.55	movement of cell or subcellular component
	GO:0046903	0.53	secretion
	GO:0030705	0.53	cytoskeleton-dependent intracellular transport
*	GO:0015780	0.53	nucleotide-sugar transmembrane transport
==== CC ====
	GO:0044424	1.00	intracellular part
	GO:0044444	0.99	cytoplasmic part
	GO:0043226	0.97	organelle
	GO:0043231	0.96	intracellular membrane-bounded organelle
	GO:0044446	0.84	intracellular organelle part
	GO:0005737	0.71	cytoplasm
	GO:0032991	0.69	protein-containing complex
*	GO:0016020	0.62	membrane
	GO:0005794	0.58	Golgi apparatus
	GO:0005634	0.53	nucleus
	GO:0044428	0.52	nuclear part
$$$$

Q5T0D9-1	phosphatidylinositol-4-phosphate phosphatase
==== MF ====
	GO:0016787	0.93	hydrolase activity
	GO:0042803	0.92	protein homodimerization activity
	GO:0016791	0.92	phosphatase activity
*	GO:0034596	0.70	phosphatidylinositol phosphate 4-phosphatase activity
*	GO:0052833	0.68	inositol monophosphate 4-phosphatase activity
==== BP ====
	GO:0065007	0.96	biological regulation
	GO:0050794	0.95	regulation of cellular process
	GO:0006810	0.94	transport
	GO:0051049	0.93	regulation of transport
	GO:2001135	0.92	regulation of endocytic recycling
	GO:0072583	0.92	clathrin-dependent endocytosis
	GO:0009987	0.91	cellular process
	GO:0008152	0.81	metabolic process
	GO:0071704	0.80	organic substance metabolic process
	GO:0044238	0.80	primary metabolic process
	GO:0044237	0.80	cellular metabolic process
	GO:0019222	0.76	regulation of metabolic process
	GO:0060255	0.75	regulation of macromolecule metabolic process
	GO:0050896	0.75	response to stimulus
	GO:0048523	0.75	negative regulation of cellular process
	GO:0048518	0.75	positive regulation of biological process
	GO:0080090	0.74	regulation of primary metabolic process
	GO:0051171	0.74	regulation of nitrogen compound metabolic process
	GO:0048522	0.74	positive regulation of cellular process
	GO:0044248	0.74	cellular catabolic process
	GO:0031323	0.74	regulation of cellular metabolic process
	GO:1901575	0.73	organic substance catabolic process
	GO:0048583	0.73	regulation of response to stimulus
	GO:0032501	0.73	multicellular organismal process
	GO:0031325	0.73	positive regulation of cellular metabolic process
	GO:0023051	0.73	regulation of signaling
	GO:0010646	0.73	regulation of cell communication
	GO:0010604	0.73	positive regulation of macromolecule metabolic process
	GO:0006950	0.73	response to stress
	GO:0051246	0.72	regulation of protein metabolic process
	GO:0051239	0.72	regulation of multicellular organismal process
	GO:0051172	0.72	negative regulation of nitrogen compound metabolic process
	GO:0050793	0.72	regulation of developmental process
	GO:0031324	0.72	negative regulation of cellular metabolic process
	GO:0023056	0.72	positive regulation of signaling
	GO:0019637	0.72	organophosphate metabolic process
	GO:0010605	0.72	negative regulation of macromolecule metabolic process
	GO:0009966	0.72	regulation of signal transduction
	GO:0006796	0.72	phosphate-containing compound metabolic process
	GO:2000145	0.71	regulation of cell motility
	GO:1902531	0.71	regulation of intracellular signal transduction
	GO:0080134	0.71	regulation of response to stress
	GO:0051241	0.71	negative regulation of multicellular organismal process
	GO:0051129	0.71	negative regulation of cellular component organization
	GO:0050767	0.71	regulation of neurogenesis
	GO:0045596	0.71	negative regulation of cell differentiation
	GO:0044255	0.71	cellular lipid metabolic process
	GO:0032101	0.71	regulation of response to external stimulus
	GO:0009968	0.71	negative regulation of signal transduction
	GO:0003008	0.71	system process
	GO:0001932	0.71	regulation of protein phosphorylation
	GO:0048681	0.70	negative regulation of axon regeneration
*	GO:0048015	0.70	phosphatidylinositol-mediated signaling
*	GO:0046856	0.70	phosphatidylinositol dephosphorylation
	GO:0042532	0.70	negative regulation of tyrosine phosphorylation of STAT protein
*	GO:0031161	0.70	phosphatidylinositol catabolic process
	GO:0014898	0.70	cardiac muscle hypertrophy in response to stress
	GO:0001921	0.70	positive regulation of receptor recycling
==== CC ====
	GO:0044424	1.00	intracellular part
	GO:0005737	0.94	cytoplasm
	GO:0044444	0.63	cytoplasmic part
	GO:0043226	0.57	organelle
	GO:0043229	0.54	intracellular organelle
	GO:0043227	0.54	membrane-bounded organelle
$$$$

Q5VTQ0-1	protein ubiquitination regulation
==== MF ====
*	GO:0019899	0.52	enzyme binding
==== BP ====
	GO:0009987	0.96	cellular process
	GO:0065007	0.81	biological regulation
	GO:0050789	0.79	regulation of biological process
	GO:0050794	0.76	regulation of cellular process
	GO:0008152	0.73	metabolic process
	GO:0071704	0.72	organic substance metabolic process
	GO:0044237	0.72	cellular metabolic process
	GO:0044238	0.71	primary metabolic process
	GO:0006807	0.69	nitrogen compound metabolic process
	GO:0043170	0.67	macromolecule metabolic process
	GO:1901564	0.65	organonitrogen compound metabolic process
	GO:0044260	0.64	cellular macromolecule metabolic process
	GO:0019538	0.62	protein metabolic process
	GO:0006464	0.60	cellular protein modification process
	GO:1901575	0.53	organic substance catabolic process
	GO:0044248	0.53	cellular catabolic process
	GO:0051128	0.52	regulation of cellular component organization
*	GO:0006508	0.52	proteolysis
	GO:0044265	0.51	cellular macromolecule catabolic process
	GO:0033043	0.50	regulation of organelle organization
*	GO:0016567	0.50	protein ubiquitination
*	GO:0051603	0.49	proteolysis involved in cellular protein catabolic process
*	GO:0043632	0.49	modification-dependent macromolecule catabolic process
==== CC ====
	GO:0044424	1.00	intracellular part
	GO:0044444	0.91	cytoplasmic part
	GO:0032991	0.91	protein-containing complex
	GO:0043226	0.88	organelle
	GO:0043229	0.84	intracellular organelle
	GO:0043227	0.83	membrane-bounded organelle
	GO:0005737	0.82	cytoplasm
	GO:0043231	0.81	intracellular membrane-bounded organelle
	GO:0044422	0.78	organelle part
	GO:0044446	0.77	intracellular organelle part
	GO:0005634	0.74	nucleus
	GO:1902494	0.71	catalytic complex
	GO:0044428	0.68	nuclear part
	GO:0005829	0.64	cytosol
	GO:1990234	0.53	transferase complex
$$$$

Q6AI39-1	sodium:potassium ion transporter
==== MF ====
	GO:0097159	0.79	organic cyclic compound binding
	GO:1901363	0.78	heterocyclic compound binding
	GO:0043167	0.65	ion binding
	GO:0016787	0.63	hydrolase activity
	GO:0043168	0.61	anion binding
	GO:0097367	0.59	carbohydrate derivative binding
	GO:0036094	0.59	small molecule binding
	GO:0016462	0.59	pyrophosphatase activity
	GO:0017111	0.58	nucleoside-triphosphatase activity
	GO:0016887	0.57	ATPase activity
	GO:0042623	0.56	ATPase activity, coupled
	GO:0022857	0.56	transmembrane transporter activity
	GO:0046873	0.55	metal ion transmembrane transporter activity
*	GO:0005391	0.54	sodium:potassium-exchanging ATPase activity
==== BP ====
	GO:0009987	0.95	cellular process
	GO:0065007	0.90	biological regulation
	GO:0050789	0.86	regulation of biological process
	GO:0050794	0.75	regulation of cellular process
	GO:0051179	0.67	localization
	GO:0006810	0.63	transport
	GO:0019222	0.58	regulation of metabolic process
	GO:0065008	0.57	regulation of biological quality
	GO:0060255	0.55	regulation of macromolecule metabolic process
	GO:0031323	0.55	regulation of cellular metabolic process
	GO:0006811	0.55	ion transport
*	GO:0010248	0.54	establishment or maintenance of transmembrane electrochemical gradient
	GO:0080090	0.53	regulation of primary metabolic process
	GO:0051171	0.52	regulation of nitrogen compound metabolic process
	GO:0010468	0.52	regulation of gene expression
	GO:0071704	0.50	organic substance metabolic process
==== CC ====
	GO:0044464	1.00	cell part
	GO:0044424	0.96	intracellular part
	GO:0044425	0.85	membrane part
	GO:0016021	0.81	integral component of membrane
	GO:0043226	0.75	organelle
	GO:0016020	0.75	membrane
	GO:0044422	0.74	organelle part
	GO:0043229	0.73	intracellular organelle
	GO:0044446	0.72	intracellular organelle part
	GO:0043227	0.69	membrane-bounded organelle
	GO:0043231	0.65	intracellular membrane-bounded organelle
	GO:0032991	0.65	protein-containing complex
	GO:0044444	0.59	cytoplasmic part
*	GO:0005886	0.55	plasma membrane
	GO:0005634	0.55	nucleus
	GO:0044428	0.52	nuclear part
$$$$

Q6ZNE9-2	regulation of protein folding
==== MF ====
*	GO:0051082	0.44	unfolded protein binding
*	GO:0044183	0.44	protein binding involved in protein folding
	GO:0019899	0.42	enzyme binding
	GO:0051020	0.40	GTPase binding
==== BP ====
	GO:0009987	0.96	cellular process
	GO:0065007	0.78	biological regulation
	GO:0071704	0.76	organic substance metabolic process
	GO:0044238	0.76	primary metabolic process
	GO:0044237	0.76	cellular metabolic process
	GO:0050794	0.75	regulation of cellular process
	GO:0006807	0.75	nitrogen compound metabolic process
	GO:0006139	0.74	nucleobase-containing compound metabolic process
	GO:0034654	0.71	nucleobase-containing compound biosynthetic process
	GO:0051179	0.69	localization
	GO:0051128	0.68	regulation of cellular component organization
	GO:0006810	0.68	transport
	GO:0055086	0.63	nucleobase-containing small molecule metabolic process
	GO:0051641	0.63	cellular localization
	GO:0006793	0.63	phosphorus metabolic process
	GO:0046907	0.57	intracellular transport
	GO:0031323	0.54	regulation of cellular metabolic process
	GO:1901135	0.53	carbohydrate derivative metabolic process
*	GO:0061077	0.51	chaperone-mediated protein folding
*	GO:0006458	0.51	'de novo' protein folding
	GO:0033043	0.49	regulation of organelle organization
	GO:0051640	0.46	organelle localization
	GO:0048518	0.46	positive regulation of biological process
	GO:0051656	0.45	establishment of organelle localization
	GO:0048522	0.45	positive regulation of cellular process
	GO:1901564	0.43	organonitrogen compound metabolic process
	GO:0051130	0.43	positive regulation of cellular component organization
	GO:1901137	0.41	carbohydrate derivative biosynthetic process
==== CC ====
	GO:0044446	1.00	intracellular organelle part
	GO:0043231	1.00	intracellular membrane-bounded organelle
	GO:0044444	0.98	cytoplasmic part
	GO:0005737	0.76	cytoplasm
	GO:0005829	0.72	cytosol
	GO:0032991	0.68	protein-containing complex
	GO:0005634	0.66	nucleus
	GO:0016020	0.58	membrane
	GO:0031982	0.54	vesicle
	GO:0031410	0.48	cytoplasmic vesicle
	GO:0005768	0.46	endosome
	GO:0044428	0.45	nuclear part
$$$$

Q6ZW76-1	transferase acting on a protein
==== MF ====
*	GO:0140096	0.52	catalytic activity, acting on a protein
*	GO:0016740	0.51	transferase activity
==== BP ====
	GO:0009987	0.95	cellular process
	GO:0065007	0.86	biological regulation
	GO:0050789	0.83	regulation of biological process
	GO:0050794	0.81	regulation of cellular process
	GO:0044237	0.58	cellular metabolic process
	GO:0071704	0.57	organic substance metabolic process
	GO:0044238	0.56	primary metabolic process
	GO:0006807	0.54	nitrogen compound metabolic process
	GO:0016043	0.52	cellular component organization
	GO:0019222	0.51	regulation of metabolic process
==== CC ====
	GO:0044464	1.00	cell part
	GO:0044424	0.98	intracellular part
	GO:0043226	0.86	organelle
	GO:0043229	0.85	intracellular organelle
	GO:0043227	0.82	membrane-bounded organelle
	GO:0043231	0.75	intracellular membrane-bounded organelle
	GO:0044444	0.74	cytoplasmic part
	GO:0005737	0.70	cytoplasm
	GO:0016020	0.69	membrane
	GO:0005634	0.64	nucleus
	GO:0044425	0.63	membrane part
	GO:0044422	0.62	organelle part
	GO:0044446	0.57	intracellular organelle part
	GO:0016021	0.57	integral component of membrane
	GO:0032991	0.56	protein-containing complex
$$$$

Q70Z53-1	cytoskelton organization and cytoskelton based transport 
==== MF ====
	GO:0016787	0.58	hydrolase activity
*	GO:0045503	0.48	dynein light chain binding
==== BP ====
	GO:0009987	0.95	cellular process
	GO:0016043	0.78	cellular component organization
	GO:0032502	0.77	developmental process
	GO:0006996	0.76	organelle organization
	GO:0009653	0.75	anatomical structure morphogenesis
	GO:0048598	0.74	embryonic morphogenesis
	GO:0008152	0.66	metabolic process
	GO:0071704	0.60	organic substance metabolic process
	GO:0065007	0.60	biological regulation
	GO:0044238	0.60	primary metabolic process
	GO:0044237	0.59	cellular metabolic process
	GO:0006807	0.59	nitrogen compound metabolic process
	GO:0050789	0.58	regulation of biological process
	GO:0050794	0.57	regulation of cellular process
	GO:0043170	0.56	macromolecule metabolic process
	GO:0022607	0.53	cellular component assembly
	GO:0034641	0.52	cellular nitrogen compound metabolic process
	GO:0120036	0.51	plasma membrane bounded cell projection organization
	GO:0070925	0.51	organelle assembly
	GO:0006139	0.51	nucleobase-containing compound metabolic process
	GO:0090304	0.50	nucleic acid metabolic process
*	GO:0060271	0.50	cilium assembly
*	GO:0048704	0.50	embryonic skeletal system morphogenesis
==== CC ====
	GO:0044464	1.00	cell part
	GO:0044424	0.99	intracellular part
	GO:0044425	0.80	membrane part
	GO:0043226	0.79	organelle
	GO:0016021	0.78	integral component of membrane
	GO:0043227	0.77	membrane-bounded organelle
	GO:0016020	0.76	membrane
	GO:0044422	0.75	organelle part
	GO:0044446	0.74	intracellular organelle part
	GO:0032991	0.70	protein-containing complex
	GO:0044444	0.67	cytoplasmic part
	GO:0043229	0.58	intracellular organelle
	GO:1902494	0.56	catalytic complex
	GO:0043231	0.54	intracellular membrane-bounded organelle
	GO:0031982	0.52	vesicle
	GO:0120038	0.51	plasma membrane bounded cell projection part
*	GO:0044430	0.50	cytoskeletal part
	GO:0044421	0.50	extracellular region part
	GO:0120025	0.49	plasma membrane bounded cell projection
	GO:0005615	0.49	extracellular space
*	GO:0000922	0.49	spindle pole
	GO:0097546	0.48	ciliary base
	GO:0070062	0.48	extracellular exosome
*	GO:0031021	0.48	interphase microtubule organizing center
	GO:0005929	0.48	cilium
*	GO:0005868	0.48	cytoplasmic dynein complex
*	GO:0005813	0.48	centrosome
*	GO:0000242	0.48	pericentriolar material
$$$$

Q7Z5A7-1	regulation of microglial cell activation
==== MF ====
==== BP ====
	GO:0050789	1.00	regulation of biological process
	GO:0050794	0.85	regulation of cellular process
	GO:0009987	0.84	cellular process
	GO:0048522	0.75	positive regulation of cellular process
	GO:0048583	0.73	regulation of response to stimulus
	GO:0048523	0.72	negative regulation of cellular process
	GO:0048584	0.71	positive regulation of response to stimulus
	GO:0048585	0.67	negative regulation of response to stimulus
*	GO:0031347	0.67	regulation of defense response
*	GO:0002682	0.67	regulation of immune system process
*	GO:1903980	0.66	positive regulation of microglial cell activation
*	GO:1903979	0.66	negative regulation of microglial cell activation
==== CC ====
	GO:0044464	0.95	cell part
	GO:0044424	0.85	intracellular part
	GO:0044421	0.74	extracellular region part
	GO:0005615	0.73	extracellular space
	GO:0043226	0.67	organelle
	GO:0043229	0.66	intracellular organelle
	GO:0044444	0.62	cytoplasmic part
	GO:0043227	0.62	membrane-bounded organelle
	GO:0043231	0.60	intracellular membrane-bounded organelle
	GO:0005783	0.50	endoplasmic reticulum
$$$$

Q8IUW5-1	regulation of apoptosis through TNF
==== MF ====
*	GO:0005031	0.40	tumor necrosis factor-activated receptor activity
==== BP ====
	GO:0050789	1.00	regulation of biological process
	GO:0009987	0.70	cellular process
	GO:0050794	0.65	regulation of cellular process
	GO:0048518	0.64	positive regulation of biological process
*	GO:0097190	0.51	apoptotic signaling pathway
*	GO:0042981	0.51	regulation of apoptotic process
*	GO:0042127	0.51	regulation of cell proliferation
	GO:0032496	0.51	response to lipopolysaccharide
	GO:0007275	0.51	multicellular organism development
*	GO:0006955	0.51	immune response
*	GO:0006954	0.51	inflammatory response
==== CC ====
	GO:0044464	1.00	cell part
	GO:0044424	0.80	intracellular part
	GO:0043229	0.76	intracellular organelle
$$$$

Q8IUY3-1	binding of GTPase from Ras superfamily
==== MF ====
	GO:0098772	0.64	molecular function regulator
*	GO:0017016	0.63	Ras GTPase binding
*	GO:0005096	0.62	GTPase activator activity
*	GO:0017137	0.61	Rab GTPase binding
==== BP ====
	GO:0065007	0.85	biological regulation
	GO:0051179	0.84	localization
	GO:0051641	0.83	cellular localization
	GO:0050794	0.82	regulation of cellular process
	GO:0071702	0.81	organic substance transport
	GO:0046907	0.81	intracellular transport
	GO:0050790	0.79	regulation of catalytic activity
	GO:0043085	0.78	positive regulation of catalytic activity
	GO:0051336	0.77	regulation of hydrolase activity
	GO:0051345	0.76	positive regulation of hydrolase activity
	GO:0032879	0.76	regulation of localization
	GO:0051128	0.74	regulation of cellular component organization
*	GO:0043087	0.74	regulation of GTPase activity
*	GO:0090630	0.73	activation of GTPase activity
	GO:0031338	0.73	regulation of vesicle fusion
	GO:0006886	0.73	intracellular protein transport
==== CC ====
	GO:0044464	0.96	cell part
	GO:0012505	0.56	endomembrane system
	GO:0005622	0.56	intracellular
$$$$

Q8N957-1	nucleotide excision repair
==== MF ====
==== BP ====
	GO:0009987	0.97	cellular process
	GO:0065007	0.85	biological regulation
	GO:0050789	0.83	regulation of biological process
	GO:0050794	0.81	regulation of cellular process
	GO:0032502	0.77	developmental process
	GO:0016043	0.73	cellular component organization
	GO:0008152	0.61	metabolic process
	GO:0044237	0.59	cellular metabolic process
	GO:0071704	0.58	organic substance metabolic process
	GO:0044238	0.56	primary metabolic process
	GO:0006807	0.56	nitrogen compound metabolic process
	GO:0048518	0.55	positive regulation of biological process
	GO:0050896	0.54	response to stimulus
	GO:0043170	0.53	macromolecule metabolic process
	GO:0044260	0.50	cellular macromolecule metabolic process
==== CC ====
	GO:0044464	1.00	cell part
	GO:0044424	0.98	intracellular part
	GO:0032991	0.86	protein-containing complex
	GO:0043226	0.76	organelle
	GO:0044422	0.75	organelle part
	GO:0043229	0.73	intracellular organelle
	GO:0044446	0.70	intracellular organelle part
	GO:0044444	0.68	cytoplasmic part
	GO:0043227	0.64	membrane-bounded organelle
	GO:0043231	0.59	intracellular membrane-bounded organelle
	GO:0005737	0.56	cytoplasm
	GO:0005634	0.50	nucleus
*	GO:1902494	0.48	catalytic complex
*	GO:0009380	0.44	excinuclease repair complex
$$$$

Q8NDM7-1	
==== MF ====
==== BP ====
	GO:0009987	0.98	cellular process
	GO:0065007	0.70	biological regulation
	GO:0050789	0.68	regulation of biological process
	GO:0050794	0.66	regulation of cellular process
	GO:0071840	0.61	cellular component organization or biogenesis
	GO:0016043	0.60	cellular component organization
	GO:0044237	0.50	cellular metabolic process
==== CC ====
	GO:0044464	0.99	cell part
	GO:0044424	0.98	intracellular part
	GO:0044446	0.81	intracellular organelle part
	GO:0043226	0.74	organelle
	GO:0043229	0.68	intracellular organelle
	GO:0044444	0.65	cytoplasmic part
	GO:0043227	0.63	membrane-bounded organelle
	GO:0032991	0.63	protein-containing complex
	GO:0043231	0.54	intracellular membrane-bounded organelle
$$$$

Q8TDG2-1	regulation of chromosome organization either though histone acetylation or binding of cytoskelton used in chromsome segregation
==== MF ====
	GO:0005198	0.90	structural molecule activity
	GO:1901363	0.89	heterocyclic compound binding
	GO:0097159	0.89	organic cyclic compound binding
	GO:0005200	0.89	structural constituent of cytoskeleton
	GO:0043167	0.85	ion binding
	GO:0035639	0.84	purine ribonucleoside triphosphate binding
	GO:0032555	0.84	purine ribonucleotide binding
	GO:0016740	0.66	transferase activity
*	GO:0004402	0.61	histone acetyltransferase activity
*	GO:0008092	0.59	cytoskeletal protein binding
==== BP ====
	GO:0065007	0.96	biological regulation
	GO:0050789	0.95	regulation of biological process
	GO:0009987	0.90	cellular process
	GO:0060255	0.89	regulation of macromolecule metabolic process
	GO:0048518	0.89	positive regulation of biological process
	GO:0010468	0.88	regulation of gene expression
	GO:0009893	0.85	positive regulation of metabolic process
	GO:0016043	0.78	cellular component organization
	GO:0010604	0.78	positive regulation of macromolecule metabolic process
	GO:0010628	0.77	positive regulation of gene expression
	GO:0032501	0.76	multicellular organismal process
	GO:0050794	0.74	regulation of cellular process
	GO:0090131	0.71	mesenchyme migration
	GO:0032502	0.71	developmental process
	GO:0044237	0.70	cellular metabolic process
	GO:0071704	0.69	organic substance metabolic process
	GO:0044238	0.69	primary metabolic process
	GO:0006996	0.69	organelle organization
	GO:0050896	0.68	response to stimulus
	GO:0006807	0.68	nitrogen compound metabolic process
	GO:1901564	0.67	organonitrogen compound metabolic process
	GO:0044260	0.67	cellular macromolecule metabolic process
	GO:0022607	0.66	cellular component assembly
	GO:0051716	0.65	cellular response to stimulus
	GO:0048869	0.65	cellular developmental process
	GO:0044267	0.65	cellular protein metabolic process
	GO:0043412	0.65	macromolecule modification
	GO:0042221	0.64	response to chemical
	GO:0006950	0.64	response to stress
	GO:0006464	0.64	cellular protein modification process
*	GO:0006325	0.64	chromatin organization
	GO:1901360	0.63	organic cyclic compound metabolic process
	GO:0034641	0.63	cellular nitrogen compound metabolic process
	GO:0022402	0.63	cell cycle process
	GO:0070887	0.62	cellular response to chemical stimulus
	GO:0033554	0.62	cellular response to stress
	GO:0022414	0.62	reproductive process
	GO:0006139	0.62	nucleobase-containing compound metabolic process
	GO:0090304	0.61	nucleic acid metabolic process
	GO:0048646	0.61	anatomical structure formation involved in morphogenesis
*	GO:0018193	0.61	peptidyl-amino acid modification
*	GO:0016570	0.61	histone modification
	GO:0003006	0.61	developmental process involved in reproduction
	GO:1903046	0.60	meiotic cell cycle process
	GO:0032506	0.60	cytokinetic process
*	GO:0016573	0.60	histone acetylation
	GO:0010927	0.60	cellular component assembly involved in morphogenesis
*	GO:0006281	0.60	DNA repair
	GO:0034599	0.59	cellular response to oxidative stress
	GO:0030476	0.59	ascospore wall assembly
	GO:0001300	0.59	chronological cell aging
*	GO:0000916	0.59	actomyosin contractile ring contraction
	GO:0000011	0.59	vacuole inheritance
	GO:0080090	0.57	regulation of primary metabolic process
	GO:0051171	0.57	regulation of nitrogen compound metabolic process
	GO:0031323	0.57	regulation of cellular metabolic process
	GO:0031326	0.54	regulation of cellular biosynthetic process
	GO:0019219	0.54	regulation of nucleobase-containing compound metabolic process
	GO:0010556	0.54	regulation of macromolecule biosynthetic process
	GO:0048522	0.53	positive regulation of cellular process
	GO:0048519	0.53	negative regulation of biological process
*	GO:0006355	0.53	regulation of transcription, DNA-templated
	GO:0051179	0.51	localization
	GO:0048523	0.51	negative regulation of cellular process
==== CC ====
	GO:0044424	1.00	intracellular part
	GO:0043226	0.94	organelle
	GO:0043229	0.93	intracellular organelle
	GO:0044446	0.88	intracellular organelle part
	GO:0032991	0.79	protein-containing complex
	GO:0044444	0.77	cytoplasmic part
	GO:0043227	0.74	membrane-bounded organelle
	GO:0005737	0.73	cytoplasm
	GO:0043231	0.71	intracellular membrane-bounded organelle
	GO:0043232	0.70	intracellular non-membrane-bounded organelle
*	GO:0005856	0.66	cytoskeleton
	GO:0005634	0.64	nucleus
*	GO:0044430	0.60	cytoskeletal part
	GO:0042995	0.54	cell projection
	GO:0016020	0.53	membrane
$$$$

Q8WTR8-1	anatomical structure morphogenesis
==== MF ====
==== BP ====
	GO:0009987	0.98	cellular process
	GO:0065007	0.88	biological regulation
	GO:0050789	0.86	regulation of biological process
	GO:0032502	0.86	developmental process
	GO:0050794	0.84	regulation of cellular process
	GO:0016043	0.71	cellular component organization
	GO:0006928	0.68	movement of cell or subcellular component
*	GO:0048856	0.66	anatomical structure development
*	GO:0009653	0.63	anatomical structure morphogenesis
	GO:0048869	0.62	cellular developmental process
	GO:0048518	0.60	positive regulation of biological process
	GO:0048522	0.55	positive regulation of cellular process
	GO:0051239	0.52	regulation of multicellular organismal process
	GO:0050793	0.52	regulation of developmental process
	GO:0051128	0.51	regulation of cellular component organization
	GO:0048519	0.51	negative regulation of biological process
	GO:2000026	0.50	regulation of multicellular organismal development
	GO:0048583	0.48	regulation of response to stimulus
*	GO:0007411	0.48	axon guidance
	GO:0045595	0.47	regulation of cell differentiation
	GO:0048523	0.46	negative regulation of cellular process
	GO:0022603	0.46	regulation of anatomical structure morphogenesis
	GO:0120035	0.45	regulation of plasma membrane bounded cell projection organization
*	GO:0051960	0.45	regulation of nervous system development
*	GO:0050767	0.44	regulation of neurogenesis
*	GO:0045664	0.43	regulation of neuron differentiation
	GO:0030030	0.43	cell projection organization
*	GO:0010975	0.42	regulation of neuron projection development
*	GO:0010769	0.41	regulation of cell morphogenesis involved in differentiation
==== CC ====
	GO:0019031	1.00	viral envelope
	GO:0044464	0.94	cell part
	GO:0044424	0.70	intracellular part
	GO:0044421	0.69	extracellular region part
	GO:0044425	0.58	membrane part
	GO:0062023	0.53	collagen-containing extracellular matrix
	GO:0044459	0.52	plasma membrane part
$$$$

Q8WXF5-1	structure component of eye lens
==== MF ====
*	GO:0005212	0.95	structural constituent of eye lens
==== BP ====
	GO:0032502	0.91	developmental process
	GO:0032501	0.86	multicellular organismal process
	GO:0003008	0.85	system process
	GO:0007601	0.84	visual perception
	GO:0009987	0.81	cellular process
	GO:0048856	0.77	anatomical structure development
	GO:0009653	0.63	anatomical structure morphogenesis
*	GO:0048513	0.59	animal organ development
	GO:0002009	0.59	morphogenesis of an epithelium
*	GO:0001654	0.58	eye development
	GO:0065007	0.49	biological regulation
*	GO:0002088	0.49	lens development in camera-type eye
==== CC ====
	GO:0005737	1.00	cytoplasm
	GO:0005634	1.00	nucleus
$$$$

Q96A25-1	lysosomal transport and lysosome organization
==== MF ====
	GO:0016787	0.44	hydrolase activity
==== BP ====
	GO:0048813	1.00	dendrite morphogenesis
*	GO:0032418	1.00	lysosome localization
	GO:0006996	0.93	organelle organization
	GO:0006810	0.93	transport
*	GO:0007041	0.92	lysosomal transport
*	GO:0007040	0.92	lysosome organization
	GO:0065007	0.64	biological regulation
	GO:0050789	0.59	regulation of biological process
	GO:0050794	0.57	regulation of cellular process
==== CC ====
	GO:0044424	1.00	intracellular part
	GO:0044444	0.98	cytoplasmic part
	GO:0016020	0.94	membrane
	GO:0044446	0.93	intracellular organelle part
	GO:0098588	0.87	bounding membrane of organelle
	GO:0044437	0.87	vacuolar part
	GO:0043226	0.87	organelle
	GO:0043229	0.86	intracellular organelle
	GO:0005774	0.86	vacuolar membrane
	GO:0043227	0.85	membrane-bounded organelle
*	GO:0005765	0.85	lysosomal membrane
	GO:0043231	0.83	intracellular membrane-bounded organelle
	GO:0005773	0.55	vacuole
	GO:0031982	0.50	vesicle
$$$$

Q96D15-1	catalytic activity, acting on a protein
==== MF ====
*	GO:0140096	0.41	catalytic activity, acting on a protein
==== BP ====
	GO:0009987	0.95	cellular process
	GO:0065007	0.83	biological regulation
	GO:0050789	0.78	regulation of biological process
	GO:0050794	0.75	regulation of cellular process
	GO:0071704	0.66	organic substance metabolic process
	GO:0044237	0.65	cellular metabolic process
	GO:0044238	0.64	primary metabolic process
	GO:0006807	0.63	nitrogen compound metabolic process
	GO:0043170	0.61	macromolecule metabolic process
	GO:0044260	0.56	cellular macromolecule metabolic process
	GO:1901564	0.54	organonitrogen compound metabolic process
	GO:0019538	0.52	protein metabolic process
	GO:0043412	0.51	macromolecule modification
==== CC ====
	GO:0044424	1.00	intracellular part
	GO:0043226	0.94	organelle
	GO:0043229	0.93	intracellular organelle
	GO:0043227	0.92	membrane-bounded organelle
	GO:0044444	0.90	cytoplasmic part
	GO:0043231	0.90	intracellular membrane-bounded organelle
	GO:0044446	0.82	intracellular organelle part
	GO:0016020	0.80	membrane
	GO:0005886	0.72	plasma membrane
	GO:0005737	0.69	cytoplasm
	GO:0044425	0.61	membrane part
	GO:0005634	0.61	nucleus
	GO:0044459	0.54	plasma membrane part
	GO:0005783	0.53	endoplasmic reticulum
$$$$

Q96F63-1	splicesome protein
==== MF ====
==== BP ====
	GO:0009987	0.83	cellular process
	GO:0008152	0.54	metabolic process
	GO:0071704	0.53	organic substance metabolic process
	GO:0044238	0.53	primary metabolic process
	GO:0044237	0.53	cellular metabolic process
	GO:0006807	0.50	nitrogen compound metabolic process
==== CC ====
*	GO:0005686	1.00	U2 snRNP
$$$$

Q96II8-1	transferase acting on a protein
==== MF ====
*	GO:0140096	0.45	catalytic activity, acting on a protein
*	GO:0016740	0.45	transferase activity
==== BP ====
	GO:0009987	0.97	cellular process
	GO:0065007	0.93	biological regulation
	GO:0050789	0.92	regulation of biological process
	GO:0050794	0.90	regulation of cellular process
==== CC ====
	GO:0044464	1.00	cell part
	GO:0016021	1.00	integral component of membrane
	GO:0044424	0.94	intracellular part
	GO:0016020	0.79	membrane
	GO:0005886	0.63	plasma membrane
	GO:0005737	0.62	cytoplasm
	GO:0043226	0.55	organelle
	GO:0044444	0.51	cytoplasmic part
	GO:0043229	0.51	intracellular organelle
$$$$

Q96J88-1	cytoskeleton binding
==== MF ====
*	GO:0008092	0.43	cytoskeletal protein binding
==== BP ====
	GO:0009987	0.95	cellular process
	GO:0065007	0.83	biological regulation
	GO:0050789	0.80	regulation of biological process
	GO:0050794	0.77	regulation of cellular process
	GO:0016043	0.73	cellular component organization
	GO:0006996	0.63	organelle organization
	GO:0048518	0.61	positive regulation of biological process
	GO:0048522	0.58	positive regulation of cellular process
*	GO:0007010	0.54	cytoskeleton organization
	GO:0022607	0.53	cellular component assembly
*	GO:0032185	0.47	septin cytoskeleton organization
*	GO:0007017	0.47	microtubule-based process
==== CC ====
	GO:0044464	1.00	cell part
	GO:0044424	0.98	intracellular part
	GO:0044444	0.84	cytoplasmic part
	GO:0043226	0.82	organelle
	GO:0043229	0.80	intracellular organelle
	GO:0044422	0.77	organelle part
	GO:0044446	0.75	intracellular organelle part
	GO:0043227	0.72	membrane-bounded organelle
	GO:0005737	0.66	cytoplasm
	GO:0043231	0.63	intracellular membrane-bounded organelle
	GO:0016020	0.62	membrane
	GO:0032991	0.59	protein-containing complex
*	GO:0044430	0.55	cytoskeletal part
	GO:0043232	0.54	intracellular non-membrane-bounded organelle
$$$$

Q96KV7-1	regulation of transcription by nucleic acid binding
==== MF ====
	GO:1901363	0.59	heterocyclic compound binding
	GO:0097159	0.59	organic cyclic compound binding
*	GO:0140110	0.46	transcription regulator activity
	GO:0003677	0.41	DNA binding
==== BP ====
	GO:0009987	0.92	cellular process
	GO:0065007	0.84	biological regulation
	GO:0050789	0.82	regulation of biological process
	GO:0050794	0.80	regulation of cellular process
	GO:0019222	0.66	regulation of metabolic process
	GO:0060255	0.64	regulation of macromolecule metabolic process
	GO:0080090	0.63	regulation of primary metabolic process
	GO:0031323	0.63	regulation of cellular metabolic process
	GO:0051171	0.62	regulation of nitrogen compound metabolic process
*	GO:0010468	0.60	regulation of gene expression
	GO:0019219	0.59	regulation of nucleobase-containing compound metabolic process
	GO:2000112	0.58	regulation of cellular macromolecule biosynthetic process
*	GO:0051252	0.58	regulation of RNA metabolic process
	GO:0071840	0.57	cellular component organization or biogenesis
*	GO:0006355	0.57	regulation of transcription, DNA-templated
	GO:0044237	0.56	cellular metabolic process
	GO:0016043	0.54	cellular component organization
	GO:0071704	0.53	organic substance metabolic process
	GO:0044238	0.53	primary metabolic process
	GO:0006807	0.53	nitrogen compound metabolic process
	GO:0043170	0.52	macromolecule metabolic process
	GO:0048519	0.46	negative regulation of biological process
	GO:0048518	0.46	positive regulation of biological process
	GO:0048523	0.44	negative regulation of cellular process
	GO:0048522	0.44	positive regulation of cellular process
*	GO:0006357	0.43	regulation of transcription by RNA polymerase II
==== CC ====
	GO:0044424	1.00	intracellular part
	GO:0043226	0.87	organelle
	GO:0043229	0.86	intracellular organelle
	GO:0043227	0.80	membrane-bounded organelle
	GO:0043231	0.78	intracellular membrane-bounded organelle
	GO:0044446	0.77	intracellular organelle part
	GO:0032991	0.75	protein-containing complex
*	GO:0005634	0.75	nucleus
*	GO:0044428	0.56	nuclear part
$$$$

Q96M27-1	protein kinase A regulation
==== MF ====
*	GO:0034237	0.65	protein kinase A regulatory subunit binding
==== BP ====
	GO:0065007	0.94	biological regulation
	GO:0050789	0.92	regulation of biological process
	GO:0050794	0.91	regulation of cellular process
	GO:0060255	0.82	regulation of macromolecule metabolic process
	GO:0031323	0.81	regulation of cellular metabolic process
	GO:0080090	0.80	regulation of primary metabolic process
	GO:0051171	0.80	regulation of nitrogen compound metabolic process
	GO:0048518	0.79	positive regulation of biological process
	GO:0048522	0.77	positive regulation of cellular process
	GO:0009987	0.77	cellular process
	GO:0065008	0.74	regulation of biological quality
	GO:0032501	0.74	multicellular organismal process
	GO:0010604	0.73	positive regulation of macromolecule metabolic process
	GO:0051173	0.72	positive regulation of nitrogen compound metabolic process
	GO:0031325	0.72	positive regulation of cellular metabolic process
	GO:0065009	0.71	regulation of molecular function
	GO:0051246	0.71	regulation of protein metabolic process
	GO:0032268	0.70	regulation of cellular protein metabolic process
	GO:0050790	0.69	regulation of catalytic activity
	GO:0031399	0.69	regulation of protein modification process
	GO:0051247	0.68	positive regulation of protein metabolic process
	GO:0044093	0.68	positive regulation of molecular function
	GO:0019220	0.68	regulation of phosphate metabolic process
	GO:0045859	0.67	regulation of protein kinase activity
	GO:0042592	0.67	homeostatic process
*	GO:0001934	0.67	positive regulation of protein phosphorylation
	GO:0043085	0.66	positive regulation of catalytic activity
	GO:0007389	0.66	pattern specification process
*	GO:0034199	0.65	activation of protein kinase A activity
	GO:0010669	0.65	epithelial structure maintenance
	GO:0001757	0.65	somite specification
==== CC ====
	GO:0044464	1.00	cell part
	GO:0044424	0.99	intracellular part
	GO:0043226	0.92	organelle
	GO:0043229	0.90	intracellular organelle
	GO:0044444	0.89	cytoplasmic part
	GO:0043227	0.88	membrane-bounded organelle
	GO:0043231	0.86	intracellular membrane-bounded organelle
	GO:0005737	0.72	cytoplasm
	GO:0005794	0.59	Golgi apparatus
	GO:0044422	0.54	organelle part
$$$$

Q96S16-1	histone demethylation
==== MF ====
	GO:0016491	0.88	oxidoreductase activity
	GO:0140096	0.87	catalytic activity, acting on a protein
	GO:0051213	0.87	dioxygenase activity
*	GO:0016705	0.87	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
*	GO:0016706	0.79	2-oxoglutarate-dependent dioxygenase activity
*	GO:0032452	0.75	histone demethylase activity
	GO:0043167	0.66	ion binding
	GO:1901363	0.65	heterocyclic compound binding
	GO:0097159	0.65	organic cyclic compound binding
	GO:0043169	0.64	cation binding
*	GO:0003682	0.50	chromatin binding
==== BP ====
	GO:0009987	0.99	cellular process
	GO:0071704	0.97	organic substance metabolic process
	GO:0044238	0.97	primary metabolic process
	GO:0044237	0.97	cellular metabolic process
	GO:1901564	0.96	organonitrogen compound metabolic process
	GO:0043170	0.96	macromolecule metabolic process
	GO:0043412	0.95	macromolecule modification
	GO:0044260	0.92	cellular macromolecule metabolic process
	GO:0019538	0.89	protein metabolic process
	GO:0006464	0.88	cellular protein modification process
	GO:0050789	0.84	regulation of biological process
	GO:0050794	0.77	regulation of cellular process
	GO:0019222	0.76	regulation of metabolic process
	GO:0080090	0.75	regulation of primary metabolic process
	GO:0060255	0.75	regulation of macromolecule metabolic process
	GO:0031323	0.75	regulation of cellular metabolic process
	GO:0051171	0.74	regulation of nitrogen compound metabolic process
	GO:0019219	0.71	regulation of nucleobase-containing compound metabolic process
	GO:0010468	0.71	regulation of gene expression
	GO:0051252	0.70	regulation of RNA metabolic process
	GO:0016043	0.67	cellular component organization
*	GO:0018193	0.63	peptidyl-amino acid modification
*	GO:0006325	0.63	chromatin organization
*	GO:0016570	0.61	histone modification
*	GO:0016577	0.52	histone demethylation
	GO:0031326	0.50	regulation of cellular biosynthetic process
==== CC ====
	GO:0044424	1.00	intracellular part
	GO:0043226	0.95	organelle
	GO:0043229	0.93	intracellular organelle
	GO:0043227	0.93	membrane-bounded organelle
	GO:0043231	0.90	intracellular membrane-bounded organelle
	GO:0044446	0.86	intracellular organelle part
*	GO:0005634	0.86	nucleus
*	GO:0044428	0.79	nuclear part
	GO:0044444	0.65	cytoplasmic part
*	GO:0005654	0.61	nucleoplasm
	GO:0032991	0.58	protein-containing complex
$$$$

Q9BZD6-1	serine-type endopeptidase
==== MF ====
	GO:0140096	0.91	catalytic activity, acting on a protein
	GO:0016787	0.90	hydrolase activity
*	GO:0070011	0.89	peptidase activity, acting on L-amino acid peptides
*	GO:0004175	0.84	endopeptidase activity
*	GO:0008236	0.79	serine-type peptidase activity
*	GO:0004252	0.74	serine-type endopeptidase activity
	GO:0043167	0.53	ion binding
==== BP ====
	GO:0065007	0.96	biological regulation
	GO:0065008	0.90	regulation of biological quality
	GO:0050789	0.87	regulation of biological process
	GO:0050878	0.82	regulation of body fluid levels
	GO:0009987	0.78	cellular process
	GO:0048583	0.77	regulation of response to stimulus
	GO:0051239	0.71	regulation of multicellular organismal process
	GO:0032101	0.64	regulation of response to external stimulus
	GO:0048519	0.63	negative regulation of biological process
	GO:0008152	0.63	metabolic process
	GO:0071704	0.62	organic substance metabolic process
	GO:0050794	0.60	regulation of cellular process
	GO:0044238	0.60	primary metabolic process
	GO:0080134	0.59	regulation of response to stress
	GO:0050818	0.59	regulation of coagulation
	GO:0048518	0.58	positive regulation of biological process
	GO:0006807	0.57	nitrogen compound metabolic process
	GO:0043170	0.56	macromolecule metabolic process
*	GO:0030193	0.56	regulation of blood coagulation
	GO:1901564	0.55	organonitrogen compound metabolic process
	GO:0051241	0.54	negative regulation of multicellular organismal process
	GO:0048585	0.54	negative regulation of response to stimulus
	GO:0032501	0.52	multicellular organismal process
	GO:0019538	0.51	protein metabolic process
==== CC ====
	GO:0044464	1.00	cell part
	GO:0044424	0.93	intracellular part
	GO:0005737	0.74	cytoplasm
	GO:0043226	0.71	organelle
	GO:0043227	0.69	membrane-bounded organelle
	GO:0044421	0.67	extracellular region part
	GO:0005615	0.65	extracellular space
	GO:0043229	0.64	intracellular organelle
	GO:0043231	0.61	intracellular membrane-bounded organelle
$$$$

Q9BZH6-1	
==== MF ====
	GO:0097159	0.60	organic cyclic compound binding
	GO:1901363	0.59	heterocyclic compound binding
==== BP ====
	GO:0009987	0.97	cellular process
	GO:0065007	0.85	biological regulation
	GO:0050789	0.82	regulation of biological process
	GO:0050794	0.80	regulation of cellular process
	GO:0044237	0.75	cellular metabolic process
	GO:0071704	0.73	organic substance metabolic process
	GO:0044238	0.73	primary metabolic process
	GO:0071840	0.72	cellular component organization or biogenesis
	GO:0006807	0.72	nitrogen compound metabolic process
	GO:0043170	0.71	macromolecule metabolic process
	GO:0016043	0.69	cellular component organization
	GO:0048518	0.63	positive regulation of biological process
	GO:0019222	0.62	regulation of metabolic process
	GO:0060255	0.61	regulation of macromolecule metabolic process
	GO:0031323	0.61	regulation of cellular metabolic process
	GO:0080090	0.60	regulation of primary metabolic process
	GO:0051171	0.60	regulation of nitrogen compound metabolic process
	GO:0048522	0.59	positive regulation of cellular process
	GO:0050896	0.58	response to stimulus
	GO:1901360	0.56	organic cyclic compound metabolic process
	GO:0044260	0.56	cellular macromolecule metabolic process
	GO:0034641	0.56	cellular nitrogen compound metabolic process
	GO:0032502	0.56	developmental process
	GO:0006725	0.56	cellular aromatic compound metabolic process
	GO:0046483	0.55	heterocycle metabolic process
	GO:0022607	0.55	cellular component assembly
	GO:0090304	0.54	nucleic acid metabolic process
	GO:0019219	0.53	regulation of nucleobase-containing compound metabolic process
	GO:0048583	0.51	regulation of response to stimulus
	GO:0009893	0.51	positive regulation of metabolic process
	GO:0051173	0.50	positive regulation of nitrogen compound metabolic process
	GO:0043933	0.50	protein-containing complex subunit organization
	GO:0031325	0.50	positive regulation of cellular metabolic process
	GO:0010604	0.50	positive regulation of macromolecule metabolic process
==== CC ====
	GO:0044424	1.00	intracellular part
	GO:0043229	0.97	intracellular organelle
	GO:0032991	0.96	protein-containing complex
	GO:0044446	0.95	intracellular organelle part
	GO:0043227	0.89	membrane-bounded organelle
	GO:0043231	0.88	intracellular membrane-bounded organelle
	GO:0044444	0.87	cytoplasmic part
	GO:0005737	0.82	cytoplasm
	GO:0044428	0.72	nuclear part
	GO:0005634	0.71	nucleus
	GO:1902494	0.57	catalytic complex
	GO:0044425	0.55	membrane part
	GO:0005829	0.50	cytosol
$$$$

Q9C0D6-1	binding of cytoskeleton
==== MF ====
*	GO:0008092	0.70	cytoskeletal protein binding
	GO:0019899	0.48	enzyme binding
	GO:0016787	0.41	hydrolase activity
==== BP ====
	GO:0009987	0.95	cellular process
	GO:0065007	0.82	biological regulation
	GO:0050789	0.80	regulation of biological process
	GO:0050794	0.75	regulation of cellular process
	GO:0016043	0.69	cellular component organization
	GO:0032502	0.54	developmental process
	GO:0048518	0.48	positive regulation of biological process
	GO:0051128	0.46	regulation of cellular component organization
	GO:0065008	0.44	regulation of biological quality
	GO:0006996	0.43	organelle organization
	GO:0022607	0.42	cellular component assembly
	GO:0048522	0.41	positive regulation of cellular process
	GO:0006928	0.40	movement of cell or subcellular component
==== CC ====
	GO:0044464	1.00	cell part
	GO:0044424	0.99	intracellular part
	GO:0032991	0.87	protein-containing complex
	GO:0044444	0.85	cytoplasmic part
	GO:0043226	0.84	organelle
	GO:0044422	0.83	organelle part
	GO:0043229	0.83	intracellular organelle
	GO:0044446	0.82	intracellular organelle part
	GO:0043227	0.72	membrane-bounded organelle
	GO:0005737	0.69	cytoplasm
	GO:0043231	0.68	intracellular membrane-bounded organelle
	GO:0016020	0.64	membrane
	GO:0005634	0.53	nucleus
	GO:0005829	0.48	cytosol
*	GO:0044430	0.47	cytoskeletal part
	GO:0043232	0.44	intracellular non-membrane-bounded organelle
	GO:0044428	0.43	nuclear part
	GO:0044425	0.40	membrane part
$$$$

Q9GZU8-1	hydrolase, probably hydrolase of protein
==== MF ====
	GO:0043167	0.54	ion binding
*	GO:0016787	0.53	hydrolase activity
*	GO:0140096	0.48	catalytic activity, acting on a protein
==== BP ====
	GO:0009987	0.88	cellular process
	GO:0065007	0.82	biological regulation
	GO:0050789	0.80	regulation of biological process
	GO:0050794	0.79	regulation of cellular process
	GO:0008152	0.70	metabolic process
	GO:0071704	0.67	organic substance metabolic process
	GO:0044238	0.67	primary metabolic process
	GO:0006807	0.65	nitrogen compound metabolic process
	GO:0043170	0.61	macromolecule metabolic process
	GO:1901564	0.55	organonitrogen compound metabolic process
	GO:0071840	0.53	cellular component organization or biogenesis
	GO:0048519	0.52	negative regulation of biological process
	GO:0016043	0.52	cellular component organization
	GO:0048523	0.51	negative regulation of cellular process
	GO:0044237	0.51	cellular metabolic process
*	GO:0019538	0.50	protein metabolic process
==== CC ====
	GO:0044464	0.99	cell part
	GO:0044424	0.98	intracellular part
	GO:0044422	0.73	organelle part
	GO:0005576	0.69	extracellular region
	GO:0044446	0.68	intracellular organelle part
	GO:0043226	0.67	organelle
	GO:0043229	0.66	intracellular organelle
	GO:0044444	0.58	cytoplasmic part
	GO:0043227	0.58	membrane-bounded organelle
	GO:0043231	0.55	intracellular membrane-bounded organelle
	GO:0016020	0.54	membrane
	GO:0044425	0.51	membrane part
$$$$

Q9H5V9-1	mRNA splicing
==== MF ====
==== BP ====
*	GO:0000398	1.00	mRNA splicing, via spliceosome
==== CC ====
	GO:0044424	1.00	intracellular part
*	GO:1902494	0.74	catalytic complex
*	GO:0071013	0.70	catalytic step 2 spliceosome
$$$$

Q9H9L7-1	by binding to RNA polymerase, regulate expression of genes such as cytokines
==== MF ====
*	GO:0019899	0.77	enzyme binding
==== BP ====
*	GO:0045944	1.00	positive regulation of transcription by RNA polymerase II
	GO:0050896	0.91	response to stimulus
	GO:0009605	0.90	response to external stimulus
	GO:0043207	0.89	response to external biotic stimulus
	GO:0009987	0.85	cellular process
	GO:1901700	0.71	response to oxygen-containing compound
	GO:0051239	0.71	regulation of multicellular organismal process
	GO:0010033	0.71	response to organic substance
	GO:0051240	0.70	positive regulation of multicellular organismal process
	GO:0033993	0.69	response to lipid
	GO:0032496	0.68	response to lipopolysaccharide
*	GO:0001819	0.68	positive regulation of cytokine production
*	GO:0032755	0.67	positive regulation of interleukin-6 production
	GO:0032502	0.65	developmental process
	GO:0048856	0.61	anatomical structure development
==== CC ====
*	GO:0005634	1.00	nucleus
	GO:0032991	0.90	protein-containing complex
	GO:0044422	0.76	organelle part
	GO:0044446	0.75	intracellular organelle part
	GO:0017053	0.65	transcriptional repressor complex
	GO:0044428	0.58	nuclear part
	GO:0044444	0.52	cytoplasmic part
$$$$

Q9NVP4-1	
==== MF ====
==== BP ====
	GO:0009987	0.93	cellular process
	GO:0065007	0.85	biological regulation
	GO:0050789	0.80	regulation of biological process
	GO:0050794	0.79	regulation of cellular process
	GO:0032502	0.51	developmental process
	GO:0016043	0.50	cellular component organization
==== CC ====
	GO:0044464	1.00	cell part
	GO:0044424	0.98	intracellular part
	GO:0043226	0.79	organelle
	GO:0043229	0.78	intracellular organelle
	GO:0005737	0.72	cytoplasm
	GO:0043227	0.69	membrane-bounded organelle
	GO:0044444	0.68	cytoplasmic part
	GO:0043231	0.63	intracellular membrane-bounded organelle
	GO:0044422	0.56	organelle part
	GO:0044446	0.52	intracellular organelle part
$$$$